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Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods

Rice fields host a large diversity of arthropods, but investigating their population dynamics and interactions is challenging. Here we describe the modification and application of a leaf blower-vac for suction sampling of arthropod populations in rice. When used in combination with an enclosure, app...

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Autores principales: Zou, Yi, van Telgen, Mario D., Chen, Junhui, Xiao, Haijun, de Kraker, Joop, Bianchi, Felix J. J. A., van der Werf, Wopke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091776/
https://www.ncbi.nlm.nih.gov/pubmed/27584040
http://dx.doi.org/10.3791/54655
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author Zou, Yi
van Telgen, Mario D.
Chen, Junhui
Xiao, Haijun
de Kraker, Joop
Bianchi, Felix J. J. A.
van der Werf, Wopke
author_facet Zou, Yi
van Telgen, Mario D.
Chen, Junhui
Xiao, Haijun
de Kraker, Joop
Bianchi, Felix J. J. A.
van der Werf, Wopke
author_sort Zou, Yi
collection PubMed
description Rice fields host a large diversity of arthropods, but investigating their population dynamics and interactions is challenging. Here we describe the modification and application of a leaf blower-vac for suction sampling of arthropod populations in rice. When used in combination with an enclosure, application of this sampling device provides absolute estimates of the populations of arthropods as numbers per standardized sampling area. The sampling efficiency depends critically on the sampling duration. In a mature rice crop, a two-minute sampling in an enclosure of 0.13 m(2) yields more than 90% of the arthropod population. The device also allows sampling of arthropods dwelling on the water surface or the soil in rice paddies, but it is not suitable for sampling fast flying insects, such as predatory Odonata or larger hymenopterous parasitoids. The modified blower-vac is simple to construct, and cheaper and easier to handle than traditional suction sampling devices, such as D-vac. The low cost makes the modified blower-vac also accessible to researchers in developing countries.
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spelling pubmed-50917762016-11-15 Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods Zou, Yi van Telgen, Mario D. Chen, Junhui Xiao, Haijun de Kraker, Joop Bianchi, Felix J. J. A. van der Werf, Wopke J Vis Exp Environmental Sciences Rice fields host a large diversity of arthropods, but investigating their population dynamics and interactions is challenging. Here we describe the modification and application of a leaf blower-vac for suction sampling of arthropod populations in rice. When used in combination with an enclosure, application of this sampling device provides absolute estimates of the populations of arthropods as numbers per standardized sampling area. The sampling efficiency depends critically on the sampling duration. In a mature rice crop, a two-minute sampling in an enclosure of 0.13 m(2) yields more than 90% of the arthropod population. The device also allows sampling of arthropods dwelling on the water surface or the soil in rice paddies, but it is not suitable for sampling fast flying insects, such as predatory Odonata or larger hymenopterous parasitoids. The modified blower-vac is simple to construct, and cheaper and easier to handle than traditional suction sampling devices, such as D-vac. The low cost makes the modified blower-vac also accessible to researchers in developing countries. MyJove Corporation 2016-08-10 /pmc/articles/PMC5091776/ /pubmed/27584040 http://dx.doi.org/10.3791/54655 Text en Copyright © 2016, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Environmental Sciences
Zou, Yi
van Telgen, Mario D.
Chen, Junhui
Xiao, Haijun
de Kraker, Joop
Bianchi, Felix J. J. A.
van der Werf, Wopke
Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods
title Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods
title_full Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods
title_fullStr Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods
title_full_unstemmed Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods
title_short Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods
title_sort modification and application of a leaf blower-vac for field sampling of arthropods
topic Environmental Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091776/
https://www.ncbi.nlm.nih.gov/pubmed/27584040
http://dx.doi.org/10.3791/54655
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