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Programmed Minichromosome Elimination as a Mechanism for Somatic Genome Reduction in Tetrahymena thermophila

The maintenance of chromosome integrity is crucial for genetic stability. However, programmed chromosome fragmentations are known to occur in many organisms, and in the ciliate Tetrahymena the five germline chromosomes are fragmented into hundreds of minichromosomes during somatic nuclear differenti...

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Autores principales: Lin, Chih-Yi Gabriela, Lin, I-Ting, Yao, Meng-Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091840/
https://www.ncbi.nlm.nih.gov/pubmed/27806059
http://dx.doi.org/10.1371/journal.pgen.1006403
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author Lin, Chih-Yi Gabriela
Lin, I-Ting
Yao, Meng-Chao
author_facet Lin, Chih-Yi Gabriela
Lin, I-Ting
Yao, Meng-Chao
author_sort Lin, Chih-Yi Gabriela
collection PubMed
description The maintenance of chromosome integrity is crucial for genetic stability. However, programmed chromosome fragmentations are known to occur in many organisms, and in the ciliate Tetrahymena the five germline chromosomes are fragmented into hundreds of minichromosomes during somatic nuclear differentiation. Here, we showed that there are different fates of these minichromosomes after chromosome breakage. Among the 326 somatic minichromosomes identified using genomic data, 50 are selectively eliminated from the mature somatic genome. Interestingly, many and probably most of these minichromosomes are eliminated during the growth period between 6 and 20 doublings right after conjugation. Genes with potential conjugation-specific functions are found in these minichromosomes. This study revealed a new mode of programmed DNA elimination in ciliates similar to those observed in parasitic nematodes, which could play a role in developmental gene regulation.
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spelling pubmed-50918402016-11-15 Programmed Minichromosome Elimination as a Mechanism for Somatic Genome Reduction in Tetrahymena thermophila Lin, Chih-Yi Gabriela Lin, I-Ting Yao, Meng-Chao PLoS Genet Research Article The maintenance of chromosome integrity is crucial for genetic stability. However, programmed chromosome fragmentations are known to occur in many organisms, and in the ciliate Tetrahymena the five germline chromosomes are fragmented into hundreds of minichromosomes during somatic nuclear differentiation. Here, we showed that there are different fates of these minichromosomes after chromosome breakage. Among the 326 somatic minichromosomes identified using genomic data, 50 are selectively eliminated from the mature somatic genome. Interestingly, many and probably most of these minichromosomes are eliminated during the growth period between 6 and 20 doublings right after conjugation. Genes with potential conjugation-specific functions are found in these minichromosomes. This study revealed a new mode of programmed DNA elimination in ciliates similar to those observed in parasitic nematodes, which could play a role in developmental gene regulation. Public Library of Science 2016-11-02 /pmc/articles/PMC5091840/ /pubmed/27806059 http://dx.doi.org/10.1371/journal.pgen.1006403 Text en © 2016 Lin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lin, Chih-Yi Gabriela
Lin, I-Ting
Yao, Meng-Chao
Programmed Minichromosome Elimination as a Mechanism for Somatic Genome Reduction in Tetrahymena thermophila
title Programmed Minichromosome Elimination as a Mechanism for Somatic Genome Reduction in Tetrahymena thermophila
title_full Programmed Minichromosome Elimination as a Mechanism for Somatic Genome Reduction in Tetrahymena thermophila
title_fullStr Programmed Minichromosome Elimination as a Mechanism for Somatic Genome Reduction in Tetrahymena thermophila
title_full_unstemmed Programmed Minichromosome Elimination as a Mechanism for Somatic Genome Reduction in Tetrahymena thermophila
title_short Programmed Minichromosome Elimination as a Mechanism for Somatic Genome Reduction in Tetrahymena thermophila
title_sort programmed minichromosome elimination as a mechanism for somatic genome reduction in tetrahymena thermophila
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091840/
https://www.ncbi.nlm.nih.gov/pubmed/27806059
http://dx.doi.org/10.1371/journal.pgen.1006403
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