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A Step by Step Protocol for Subretinal Surgery in Rabbits
Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE related diseases are the most common causes for irreversible loss of vision in adults in industrially developed countries. RPE transplantation appears to be a promising therapy, as it may replace dysfunctional RPE, restore i...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5092014/ https://www.ncbi.nlm.nih.gov/pubmed/27684952 http://dx.doi.org/10.3791/53927 |
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author | Al-Nawaiseh, Sami Thieltges, Fabian Liu, Zengping Strack, Claudine Brinken, Ralf Braun, Norbert Wolschendorf, Marc Maminishkis, Arvydas Eter, Nicole Stanzel, Boris V. |
author_facet | Al-Nawaiseh, Sami Thieltges, Fabian Liu, Zengping Strack, Claudine Brinken, Ralf Braun, Norbert Wolschendorf, Marc Maminishkis, Arvydas Eter, Nicole Stanzel, Boris V. |
author_sort | Al-Nawaiseh, Sami |
collection | PubMed |
description | Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE related diseases are the most common causes for irreversible loss of vision in adults in industrially developed countries. RPE transplantation appears to be a promising therapy, as it may replace dysfunctional RPE, restore its function, and thereby vision. Here we describe a method for transplanting a cultured RPE monolayer on a scaffold into the subretinal space (SRS) of rabbits. After vitrectomy xenotransplants were delivered into the SRS using a custom made shooter consisting of a 20-gauge metallic nozzle with a polytetrafluoroethylene (PTFE) coated plunger. The current technique evolved in over 150 rabbit surgeries over 6 years. Post-operative follow-up can be obtained using non-invasive and repetitive in vivo imaging such as spectral domain optical coherence tomography (SD-OCT) followed by perfusion-fixed histology. The method has well-defined steps for easy learning and high success rate. Rabbits are considered a large eye animal model useful in preclinical studies for clinical translation. In this context rabbits are a cost-efficient and perhaps convenient alternative to other large eye animal models. |
format | Online Article Text |
id | pubmed-5092014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-50920142016-11-15 A Step by Step Protocol for Subretinal Surgery in Rabbits Al-Nawaiseh, Sami Thieltges, Fabian Liu, Zengping Strack, Claudine Brinken, Ralf Braun, Norbert Wolschendorf, Marc Maminishkis, Arvydas Eter, Nicole Stanzel, Boris V. J Vis Exp Medicine Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE related diseases are the most common causes for irreversible loss of vision in adults in industrially developed countries. RPE transplantation appears to be a promising therapy, as it may replace dysfunctional RPE, restore its function, and thereby vision. Here we describe a method for transplanting a cultured RPE monolayer on a scaffold into the subretinal space (SRS) of rabbits. After vitrectomy xenotransplants were delivered into the SRS using a custom made shooter consisting of a 20-gauge metallic nozzle with a polytetrafluoroethylene (PTFE) coated plunger. The current technique evolved in over 150 rabbit surgeries over 6 years. Post-operative follow-up can be obtained using non-invasive and repetitive in vivo imaging such as spectral domain optical coherence tomography (SD-OCT) followed by perfusion-fixed histology. The method has well-defined steps for easy learning and high success rate. Rabbits are considered a large eye animal model useful in preclinical studies for clinical translation. In this context rabbits are a cost-efficient and perhaps convenient alternative to other large eye animal models. MyJove Corporation 2016-09-13 /pmc/articles/PMC5092014/ /pubmed/27684952 http://dx.doi.org/10.3791/53927 Text en Copyright © 2016, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Medicine Al-Nawaiseh, Sami Thieltges, Fabian Liu, Zengping Strack, Claudine Brinken, Ralf Braun, Norbert Wolschendorf, Marc Maminishkis, Arvydas Eter, Nicole Stanzel, Boris V. A Step by Step Protocol for Subretinal Surgery in Rabbits |
title | A Step by Step Protocol for Subretinal Surgery in Rabbits |
title_full | A Step by Step Protocol for Subretinal Surgery in Rabbits |
title_fullStr | A Step by Step Protocol for Subretinal Surgery in Rabbits |
title_full_unstemmed | A Step by Step Protocol for Subretinal Surgery in Rabbits |
title_short | A Step by Step Protocol for Subretinal Surgery in Rabbits |
title_sort | step by step protocol for subretinal surgery in rabbits |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5092014/ https://www.ncbi.nlm.nih.gov/pubmed/27684952 http://dx.doi.org/10.3791/53927 |
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