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Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments

Antibodies engineered for intracellular function must not only have affinity for their target antigen, but must also be soluble and correctly folded in the cytoplasm. Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein foldin...

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Detalles Bibliográficos
Autores principales: Moghaddam-Taaheri, Parisa, Ikonomova, Svetlana P., Gong, Zifan, Wisniewski, Janna Q., Karlsson, Amy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5092199/
https://www.ncbi.nlm.nih.gov/pubmed/27805609
http://dx.doi.org/10.3791/54583