Combined (13)C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

BACKGROUND: Pichia pastoris is a popular recombinant protein expression system for its accessibility of efficient gene manipulation and high protein production. Sufficient supply of precursors, energy, and redox cofactors is crucial for high recombinant protein production. In our present work, we fo...

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Detalles Bibliográficos
Autores principales: Liu, Ping, Huang, Mingzhi, Guo, Menglei, Qian, Jiangchao, Lin, Weilu, Chu, Ju, Zhuang, Yingping, Zhang, Siliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093185/
https://www.ncbi.nlm.nih.gov/pubmed/27867835
http://dx.doi.org/10.1186/s40643-016-0124-6

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