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An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba

Purpose. To use in vitro and in vivo models to evaluate Glechoma longituba extract to provide scientific evidence for this extract's antiurolithic activity. Materials and Methods. Potassium citrate was used as a positive control group. Oxidative stress (OS) markers and the expression of osteopo...

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Autores principales: Liang, Qiang, Li, Xiaoran, Zhou, Wangning, Su, Yu, He, Shenbao, Cheng, Shuanglei, Lu, Jianzhong, Cao, Wenjuan, Yan, Yuke, Pei, Xiaxia, Qi, Jin, Xu, Guangli, Yue, Zhongjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093267/
https://www.ncbi.nlm.nih.gov/pubmed/27840669
http://dx.doi.org/10.1155/2016/3134919
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author Liang, Qiang
Li, Xiaoran
Zhou, Wangning
Su, Yu
He, Shenbao
Cheng, Shuanglei
Lu, Jianzhong
Cao, Wenjuan
Yan, Yuke
Pei, Xiaxia
Qi, Jin
Xu, Guangli
Yue, Zhongjin
author_facet Liang, Qiang
Li, Xiaoran
Zhou, Wangning
Su, Yu
He, Shenbao
Cheng, Shuanglei
Lu, Jianzhong
Cao, Wenjuan
Yan, Yuke
Pei, Xiaxia
Qi, Jin
Xu, Guangli
Yue, Zhongjin
author_sort Liang, Qiang
collection PubMed
description Purpose. To use in vitro and in vivo models to evaluate Glechoma longituba extract to provide scientific evidence for this extract's antiurolithic activity. Materials and Methods. Potassium citrate was used as a positive control group. Oxidative stress (OS) markers and the expression of osteopontin (OPN) and kidney injury molecule-1 (KIM-1) were measured to assess the protective effects of Glechoma longituba. Multiple urolithiasis-related biochemical parameters were evaluated in urine and serum. Kidneys were harvested for histological examination and the assessment of crystal deposits. Results. In vitro and in vivo experiments demonstrated that treatment with Glechoma longituba extract significantly decreased calcium oxalate- (CaOx-) induced OPN expression, KIM-1 expression, and OS compared with the positive control group (P < 0.05). Additionally, in vivo rats that received Glechoma longituba extract exhibited significantly decreased CaOx deposits and pathological alterations (P < 0.05) compared with urolithic rats. Significantly lower levels of oxalate, creatinine, and urea and increased citrate levels were observed among rats that received Glechoma longituba (P < 0.05) compared with urolithic rats. Conclusion. Glechoma longituba has antiurolithic effects due to its possible combined effects of increasing antioxidant levels, decreasing urinary stone-forming constituents and urolithiasis-related protein expression, and elevating urinary citrate levels.
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spelling pubmed-50932672016-11-13 An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba Liang, Qiang Li, Xiaoran Zhou, Wangning Su, Yu He, Shenbao Cheng, Shuanglei Lu, Jianzhong Cao, Wenjuan Yan, Yuke Pei, Xiaxia Qi, Jin Xu, Guangli Yue, Zhongjin Oxid Med Cell Longev Research Article Purpose. To use in vitro and in vivo models to evaluate Glechoma longituba extract to provide scientific evidence for this extract's antiurolithic activity. Materials and Methods. Potassium citrate was used as a positive control group. Oxidative stress (OS) markers and the expression of osteopontin (OPN) and kidney injury molecule-1 (KIM-1) were measured to assess the protective effects of Glechoma longituba. Multiple urolithiasis-related biochemical parameters were evaluated in urine and serum. Kidneys were harvested for histological examination and the assessment of crystal deposits. Results. In vitro and in vivo experiments demonstrated that treatment with Glechoma longituba extract significantly decreased calcium oxalate- (CaOx-) induced OPN expression, KIM-1 expression, and OS compared with the positive control group (P < 0.05). Additionally, in vivo rats that received Glechoma longituba extract exhibited significantly decreased CaOx deposits and pathological alterations (P < 0.05) compared with urolithic rats. Significantly lower levels of oxalate, creatinine, and urea and increased citrate levels were observed among rats that received Glechoma longituba (P < 0.05) compared with urolithic rats. Conclusion. Glechoma longituba has antiurolithic effects due to its possible combined effects of increasing antioxidant levels, decreasing urinary stone-forming constituents and urolithiasis-related protein expression, and elevating urinary citrate levels. Hindawi Publishing Corporation 2016 2016-10-20 /pmc/articles/PMC5093267/ /pubmed/27840669 http://dx.doi.org/10.1155/2016/3134919 Text en Copyright © 2016 Qiang Liang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liang, Qiang
Li, Xiaoran
Zhou, Wangning
Su, Yu
He, Shenbao
Cheng, Shuanglei
Lu, Jianzhong
Cao, Wenjuan
Yan, Yuke
Pei, Xiaxia
Qi, Jin
Xu, Guangli
Yue, Zhongjin
An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba
title An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba
title_full An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba
title_fullStr An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba
title_full_unstemmed An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba
title_short An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba
title_sort explanation of the underlying mechanisms for the in vitro and in vivo antiurolithic activity of glechoma longituba
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093267/
https://www.ncbi.nlm.nih.gov/pubmed/27840669
http://dx.doi.org/10.1155/2016/3134919
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