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In vitro membrane protein synthesis inside Sec translocon-reconstituted cell-sized liposomes

Protein synthesis using an in vitro transcription-translation system (IVTT) inside cell-sized liposomes has become a valuable tool to study the properties of biological systems under cell-mimicking conditions. However, previous liposome systems lacked the machinery for membrane protein translocation...

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Detalles Bibliográficos
Autores principales: Ohta, Naoki, Kato, Yasuhiko, Watanabe, Hajime, Mori, Hirotada, Matsuura, Tomoaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093552/
https://www.ncbi.nlm.nih.gov/pubmed/27808179
http://dx.doi.org/10.1038/srep36466