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Influence of Taper of Root Canal Shape on the Intracanal Bacterial Reduction

OBJECTIVES: Taper is a factor that determines final root canal dimensions and consequently, the space available for the cleaning action of irrigants. Therefore, the aim of this study was to investigate the influence of taper on intracanal bacterial reduction. METHODS: Sterilized root canals of 25 ma...

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Autores principales: Paraskevopoulou, Mary Th., Khabbaz, Marouan G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093865/
https://www.ncbi.nlm.nih.gov/pubmed/27857818
http://dx.doi.org/10.2174/1874210601610010568
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author Paraskevopoulou, Mary Th.
Khabbaz, Marouan G.
author_facet Paraskevopoulou, Mary Th.
Khabbaz, Marouan G.
author_sort Paraskevopoulou, Mary Th.
collection PubMed
description OBJECTIVES: Taper is a factor that determines final root canal dimensions and consequently, the space available for the cleaning action of irrigants. Therefore, the aim of this study was to investigate the influence of taper on intracanal bacterial reduction. METHODS: Sterilized root canals of 25 mandibular incisors were inoculated with E. faecalis and then divided into two experimental groups and one control group (A= saline, B= NaOCl + EDTA and C= control, not prepared). Groups A and B were prepared to an apical size of #30/0.04, a culture was obtained and then prepared to #30/0.08 and cultured again. Final irrigation sequence was 30 ml of saline for group A and 10 ml of 17% EDTA followed by 10 ml of 2.5% NaOCl and 10 ml of saline for group B. The CFU ml(-1) of the pre- and two post-operative samplings were recorded. Values were compared by performing ANOVA and FDR for multiple hypothesis testing. RESULTS: No statistically significant difference between initial CFU ml(-1) readings was recorded, whereas significant differences between group A and B at both 4% and 8% tapers were noted. Furthermore, a linear-dependent reduction of CFU ml(-1) was recorded in each group from non-instrumented root canals to #30/0.04 and subsequently to #30/0.08. The positive control group shows the expected reduction of bacterial count. CONCLUSION: Chemomechanical instrumentation was more efficient at reducing E. faecalis when the taper of root canals increased from 4% to 8% and NaOCl plus EDTA led to a greater intracanal bacterial reduction than saline, regardless of the taper achieved.
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spelling pubmed-50938652016-11-17 Influence of Taper of Root Canal Shape on the Intracanal Bacterial Reduction Paraskevopoulou, Mary Th. Khabbaz, Marouan G. Open Dent J Article OBJECTIVES: Taper is a factor that determines final root canal dimensions and consequently, the space available for the cleaning action of irrigants. Therefore, the aim of this study was to investigate the influence of taper on intracanal bacterial reduction. METHODS: Sterilized root canals of 25 mandibular incisors were inoculated with E. faecalis and then divided into two experimental groups and one control group (A= saline, B= NaOCl + EDTA and C= control, not prepared). Groups A and B were prepared to an apical size of #30/0.04, a culture was obtained and then prepared to #30/0.08 and cultured again. Final irrigation sequence was 30 ml of saline for group A and 10 ml of 17% EDTA followed by 10 ml of 2.5% NaOCl and 10 ml of saline for group B. The CFU ml(-1) of the pre- and two post-operative samplings were recorded. Values were compared by performing ANOVA and FDR for multiple hypothesis testing. RESULTS: No statistically significant difference between initial CFU ml(-1) readings was recorded, whereas significant differences between group A and B at both 4% and 8% tapers were noted. Furthermore, a linear-dependent reduction of CFU ml(-1) was recorded in each group from non-instrumented root canals to #30/0.04 and subsequently to #30/0.08. The positive control group shows the expected reduction of bacterial count. CONCLUSION: Chemomechanical instrumentation was more efficient at reducing E. faecalis when the taper of root canals increased from 4% to 8% and NaOCl plus EDTA led to a greater intracanal bacterial reduction than saline, regardless of the taper achieved. Bentham Open 2016-10-31 /pmc/articles/PMC5093865/ /pubmed/27857818 http://dx.doi.org/10.2174/1874210601610010568 Text en © Paraskevopoulou and Khabbaz; Licensee Bentham Open https://creativecommons.org/licenses/by/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Paraskevopoulou, Mary Th.
Khabbaz, Marouan G.
Influence of Taper of Root Canal Shape on the Intracanal Bacterial Reduction
title Influence of Taper of Root Canal Shape on the Intracanal Bacterial Reduction
title_full Influence of Taper of Root Canal Shape on the Intracanal Bacterial Reduction
title_fullStr Influence of Taper of Root Canal Shape on the Intracanal Bacterial Reduction
title_full_unstemmed Influence of Taper of Root Canal Shape on the Intracanal Bacterial Reduction
title_short Influence of Taper of Root Canal Shape on the Intracanal Bacterial Reduction
title_sort influence of taper of root canal shape on the intracanal bacterial reduction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093865/
https://www.ncbi.nlm.nih.gov/pubmed/27857818
http://dx.doi.org/10.2174/1874210601610010568
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