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Glycogen distribution in the microwave‐fixed mouse brain reveals heterogeneous astrocytic patterns

In the brain, glycogen metabolism has been implied in synaptic plasticity and learning, yet the distribution of this molecule has not been fully described. We investigated cerebral glycogen of the mouse by immunohistochemistry (IHC) using two monoclonal antibodies that have different affinities depe...

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Autores principales: Oe, Yuki, Baba, Otto, Ashida, Hitoshi, Nakamura, Kouichi C., Hirase, Hajime
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094520/
https://www.ncbi.nlm.nih.gov/pubmed/27353480
http://dx.doi.org/10.1002/glia.23020
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author Oe, Yuki
Baba, Otto
Ashida, Hitoshi
Nakamura, Kouichi C.
Hirase, Hajime
author_facet Oe, Yuki
Baba, Otto
Ashida, Hitoshi
Nakamura, Kouichi C.
Hirase, Hajime
author_sort Oe, Yuki
collection PubMed
description In the brain, glycogen metabolism has been implied in synaptic plasticity and learning, yet the distribution of this molecule has not been fully described. We investigated cerebral glycogen of the mouse by immunohistochemistry (IHC) using two monoclonal antibodies that have different affinities depending on the glycogen size. The use of focused microwave irradiation yielded well‐defined glycogen immunoreactive signals compared with the conventional periodic acid‐Schiff method. The IHC signals displayed a punctate distribution localized predominantly in astrocytic processes. Glycogen immunoreactivity (IR) was high in the hippocampus, striatum, cortex, and cerebellar molecular layer, whereas it was low in the white matter and most of the subcortical structures. Additionally, glycogen distribution in the hippocampal CA3‐CA1 and striatum had a ‘patchy’ appearance with glycogen‐rich and glycogen‐poor astrocytes appearing in alternation. The glycogen patches were more evident with large‐molecule glycogen in young adult mice but they were hardly observable in aged mice (1–2 years old). Our results reveal brain region‐dependent glycogen accumulation and possibly metabolic heterogeneity of astrocytes. GLIA 2016;64:1532–1545
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spelling pubmed-50945202016-11-09 Glycogen distribution in the microwave‐fixed mouse brain reveals heterogeneous astrocytic patterns Oe, Yuki Baba, Otto Ashida, Hitoshi Nakamura, Kouichi C. Hirase, Hajime Glia Research Articles In the brain, glycogen metabolism has been implied in synaptic plasticity and learning, yet the distribution of this molecule has not been fully described. We investigated cerebral glycogen of the mouse by immunohistochemistry (IHC) using two monoclonal antibodies that have different affinities depending on the glycogen size. The use of focused microwave irradiation yielded well‐defined glycogen immunoreactive signals compared with the conventional periodic acid‐Schiff method. The IHC signals displayed a punctate distribution localized predominantly in astrocytic processes. Glycogen immunoreactivity (IR) was high in the hippocampus, striatum, cortex, and cerebellar molecular layer, whereas it was low in the white matter and most of the subcortical structures. Additionally, glycogen distribution in the hippocampal CA3‐CA1 and striatum had a ‘patchy’ appearance with glycogen‐rich and glycogen‐poor astrocytes appearing in alternation. The glycogen patches were more evident with large‐molecule glycogen in young adult mice but they were hardly observable in aged mice (1–2 years old). Our results reveal brain region‐dependent glycogen accumulation and possibly metabolic heterogeneity of astrocytes. GLIA 2016;64:1532–1545 John Wiley and Sons Inc. 2016-06-29 2016-09 /pmc/articles/PMC5094520/ /pubmed/27353480 http://dx.doi.org/10.1002/glia.23020 Text en © 2016 The Authors. Glia Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Oe, Yuki
Baba, Otto
Ashida, Hitoshi
Nakamura, Kouichi C.
Hirase, Hajime
Glycogen distribution in the microwave‐fixed mouse brain reveals heterogeneous astrocytic patterns
title Glycogen distribution in the microwave‐fixed mouse brain reveals heterogeneous astrocytic patterns
title_full Glycogen distribution in the microwave‐fixed mouse brain reveals heterogeneous astrocytic patterns
title_fullStr Glycogen distribution in the microwave‐fixed mouse brain reveals heterogeneous astrocytic patterns
title_full_unstemmed Glycogen distribution in the microwave‐fixed mouse brain reveals heterogeneous astrocytic patterns
title_short Glycogen distribution in the microwave‐fixed mouse brain reveals heterogeneous astrocytic patterns
title_sort glycogen distribution in the microwave‐fixed mouse brain reveals heterogeneous astrocytic patterns
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094520/
https://www.ncbi.nlm.nih.gov/pubmed/27353480
http://dx.doi.org/10.1002/glia.23020
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