The molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum

Aflatoxin B(1) (AFB(1)) has potent hepatotoxic, carcinogenic, genotoxic, immunotoxic and other adverse effects in human and animals. The aim of this study was to investigate the molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum of broilers. Broilers, as experimental an...

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Autores principales: Yin, Heng, Jiang, Min, Peng, Xi, Cui, Hengmin, Zhou, Yi, He, Min, Zuo, Zhicai, Ouyang, Ping, Fan, Junde, Fang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper: Pathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094947/
https://www.ncbi.nlm.nih.gov/pubmed/27232757
http://dx.doi.org/10.18632/oncotarget.9594
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author Yin, Heng
Jiang, Min
Peng, Xi
Cui, Hengmin
Zhou, Yi
He, Min
Zuo, Zhicai
Ouyang, Ping
Fan, Junde
Fang, Jing
author_facet Yin, Heng
Jiang, Min
Peng, Xi
Cui, Hengmin
Zhou, Yi
He, Min
Zuo, Zhicai
Ouyang, Ping
Fan, Junde
Fang, Jing
author_sort Yin, Heng
collection PubMed
description Aflatoxin B(1) (AFB(1)) has potent hepatotoxic, carcinogenic, genotoxic, immunotoxic and other adverse effects in human and animals. The aim of this study was to investigate the molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum of broilers. Broilers, as experimental animals, were fed 0.6 mg/kg AFB(1) diet for 3 weeks. Our results showed that AFB(1) reduced the jejunal villus height, villus height/crypt ratio and caused G(2)/M cell cycle arrest. The G(2)/M cell cycle was accompanied by the increase of ataxia telangiectasia mutated (ATM), p53, Chk2, p21 protein and mRNA expression, and the decrease of Mdm2, cdc25C, cdc2, cyclin B and proliferating cell nuclear antigen protein and mRNA expression. In conclusion, AFB(1) blocked G(2)/M cell cycle by ATM pathway in the jejunum of broilers.
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spelling pubmed-50949472016-11-22 The molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum Yin, Heng Jiang, Min Peng, Xi Cui, Hengmin Zhou, Yi He, Min Zuo, Zhicai Ouyang, Ping Fan, Junde Fang, Jing Oncotarget Research Paper: Pathology Aflatoxin B(1) (AFB(1)) has potent hepatotoxic, carcinogenic, genotoxic, immunotoxic and other adverse effects in human and animals. The aim of this study was to investigate the molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum of broilers. Broilers, as experimental animals, were fed 0.6 mg/kg AFB(1) diet for 3 weeks. Our results showed that AFB(1) reduced the jejunal villus height, villus height/crypt ratio and caused G(2)/M cell cycle arrest. The G(2)/M cell cycle was accompanied by the increase of ataxia telangiectasia mutated (ATM), p53, Chk2, p21 protein and mRNA expression, and the decrease of Mdm2, cdc25C, cdc2, cyclin B and proliferating cell nuclear antigen protein and mRNA expression. In conclusion, AFB(1) blocked G(2)/M cell cycle by ATM pathway in the jejunum of broilers. Impact Journals LLC 2016-05-25 /pmc/articles/PMC5094947/ /pubmed/27232757 http://dx.doi.org/10.18632/oncotarget.9594 Text en Copyright: © 2016 Yin et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Pathology
Yin, Heng
Jiang, Min
Peng, Xi
Cui, Hengmin
Zhou, Yi
He, Min
Zuo, Zhicai
Ouyang, Ping
Fan, Junde
Fang, Jing
The molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum
title The molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum
title_full The molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum
title_fullStr The molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum
title_full_unstemmed The molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum
title_short The molecular mechanism of G(2)/M cell cycle arrest induced by AFB(1) in the jejunum
title_sort molecular mechanism of g(2)/m cell cycle arrest induced by afb(1) in the jejunum
topic Research Paper: Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094947/
https://www.ncbi.nlm.nih.gov/pubmed/27232757
http://dx.doi.org/10.18632/oncotarget.9594
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