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The Hippo transducer TAZ promotes cell proliferation and tumor formation of glioblastoma cells through EGFR pathway
TAZ, a WW-domain-containing transcriptional co-activator, is important for development of various tissues in mammals. Recently, TAZ has been found to be overexpressed in some types of human cancers. However, the role of TAZ in glioblastoma remains unclear. In this study, we found that TAZ was overex...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094998/ https://www.ncbi.nlm.nih.gov/pubmed/27167112 http://dx.doi.org/10.18632/oncotarget.9199 |
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author | Yang, Rui Wu, Yanan Zou, Jiahua Zhou, Ji Wang, Mei Hao, Xiangwei Cui, Hongjuan |
author_facet | Yang, Rui Wu, Yanan Zou, Jiahua Zhou, Ji Wang, Mei Hao, Xiangwei Cui, Hongjuan |
author_sort | Yang, Rui |
collection | PubMed |
description | TAZ, a WW-domain-containing transcriptional co-activator, is important for development of various tissues in mammals. Recently, TAZ has been found to be overexpressed in some types of human cancers. However, the role of TAZ in glioblastoma remains unclear. In this study, we found that TAZ was overexpressed in prognostically poor glioblastoma patients. Through knocking down or overexpressing TAZ in U87 and LN229 cells, the expression level of TAZ was found to be positively related to cell proliferation in vitro and tumor formation in vivo. Further investigation indicated that TAZ could significantly promote the acceleration of cell cycle. Moreover, the western blot for p-EGFR, p-AKT, p-ERK1/2, p21, cyclin E and CDK2 proteins, target genes of the EGFR pathway, indicated that TAZ significantly activated EGFR/AKT/ERK signaling. Additionally, the blockage of EGFR pathway resulted in a significantly inhibition of cell proliferation induced by TAZ. Taken together, these results demonstrate that TAZ can promote proliferation and tumor formation in glioblastoma cells by potentiating the EGFR/AKT/ERK pathway, and provide the evidence for promising target for the treatment of glioblastoma. |
format | Online Article Text |
id | pubmed-5094998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-50949982016-11-22 The Hippo transducer TAZ promotes cell proliferation and tumor formation of glioblastoma cells through EGFR pathway Yang, Rui Wu, Yanan Zou, Jiahua Zhou, Ji Wang, Mei Hao, Xiangwei Cui, Hongjuan Oncotarget Research Paper TAZ, a WW-domain-containing transcriptional co-activator, is important for development of various tissues in mammals. Recently, TAZ has been found to be overexpressed in some types of human cancers. However, the role of TAZ in glioblastoma remains unclear. In this study, we found that TAZ was overexpressed in prognostically poor glioblastoma patients. Through knocking down or overexpressing TAZ in U87 and LN229 cells, the expression level of TAZ was found to be positively related to cell proliferation in vitro and tumor formation in vivo. Further investigation indicated that TAZ could significantly promote the acceleration of cell cycle. Moreover, the western blot for p-EGFR, p-AKT, p-ERK1/2, p21, cyclin E and CDK2 proteins, target genes of the EGFR pathway, indicated that TAZ significantly activated EGFR/AKT/ERK signaling. Additionally, the blockage of EGFR pathway resulted in a significantly inhibition of cell proliferation induced by TAZ. Taken together, these results demonstrate that TAZ can promote proliferation and tumor formation in glioblastoma cells by potentiating the EGFR/AKT/ERK pathway, and provide the evidence for promising target for the treatment of glioblastoma. Impact Journals LLC 2016-05-06 /pmc/articles/PMC5094998/ /pubmed/27167112 http://dx.doi.org/10.18632/oncotarget.9199 Text en Copyright: © 2016 Yang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Yang, Rui Wu, Yanan Zou, Jiahua Zhou, Ji Wang, Mei Hao, Xiangwei Cui, Hongjuan The Hippo transducer TAZ promotes cell proliferation and tumor formation of glioblastoma cells through EGFR pathway |
title | The Hippo transducer TAZ promotes cell proliferation and tumor formation of glioblastoma cells through EGFR pathway |
title_full | The Hippo transducer TAZ promotes cell proliferation and tumor formation of glioblastoma cells through EGFR pathway |
title_fullStr | The Hippo transducer TAZ promotes cell proliferation and tumor formation of glioblastoma cells through EGFR pathway |
title_full_unstemmed | The Hippo transducer TAZ promotes cell proliferation and tumor formation of glioblastoma cells through EGFR pathway |
title_short | The Hippo transducer TAZ promotes cell proliferation and tumor formation of glioblastoma cells through EGFR pathway |
title_sort | hippo transducer taz promotes cell proliferation and tumor formation of glioblastoma cells through egfr pathway |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5094998/ https://www.ncbi.nlm.nih.gov/pubmed/27167112 http://dx.doi.org/10.18632/oncotarget.9199 |
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