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Alteration of NPY in hypothalamus and its correlation with leptin and ghrelin during the development of T2DM in a rat model
OBJECTIVES: This study aimed to investigate the alteration of Neuropeptide Y (NPY) in the hypothalamus and its correlation with insulin, leptin and ghrelin during the development of a rat model of type 2 diabetes mellitus. METHODS: The type 2 diabetes mellitus model was developed in diet-induced obe...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095108/ https://www.ncbi.nlm.nih.gov/pubmed/27867820 http://dx.doi.org/10.1186/s40064-016-3555-9 |
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author | Zhang, Qing-jiu Yang, Chang-chun Zhang, Song-yun Zhang, Li-hui Li, Jie |
author_facet | Zhang, Qing-jiu Yang, Chang-chun Zhang, Song-yun Zhang, Li-hui Li, Jie |
author_sort | Zhang, Qing-jiu |
collection | PubMed |
description | OBJECTIVES: This study aimed to investigate the alteration of Neuropeptide Y (NPY) in the hypothalamus and its correlation with insulin, leptin and ghrelin during the development of a rat model of type 2 diabetes mellitus. METHODS: The type 2 diabetes mellitus model was developed in diet-induced obesity (DIO) rats followed by the intraperitoneal injection of low-dose streptozotocin (STZ, 25 mg/kg). At four time points during the development of type 2 diabetes mellitus in rats, the fasting serum insulin, leptin, and plasma ghrelin were measured and the hypothalamic neuropeptide Y (NPY) content and mRNA expression were detected in the rats, which were divided into 4 groups: normal control (NC), DIO(4W), DIO(8W), and T2DM; the mRNA expression of OB-Rb, and GSH-R1a in the hypothalamus were also assayed. RESULTS: During the development of the type 2 diabetes mellitus rat model, both the fasting serum levels of insulin and leptin (ng/ml) elevated significantly and the fasting plasma ghrelin concentration decreased the hypothalamic NPY (pg/mg) content significantly. NPY mRNA increased significantly in a time-dependent fashion while both the OB-Rb and the GHS-R1a mRNA of the hypothalamus decreased significantly. Hypothalamic NPY concentration was positively correlated with the changes in serum insulin and leptin and negatively correlated with plasma ghrelin. CONCLUSIONS: During the development of the type 2 diabetes mellitus rat model, the hypothalamic NPY content and NPY mRNA expression increased in a time-dependent manner, which was positively correlated with the changes of the serum insulin and leptin and negatively correlated with the plasma ghrelin. |
format | Online Article Text |
id | pubmed-5095108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-50951082016-11-18 Alteration of NPY in hypothalamus and its correlation with leptin and ghrelin during the development of T2DM in a rat model Zhang, Qing-jiu Yang, Chang-chun Zhang, Song-yun Zhang, Li-hui Li, Jie Springerplus Research OBJECTIVES: This study aimed to investigate the alteration of Neuropeptide Y (NPY) in the hypothalamus and its correlation with insulin, leptin and ghrelin during the development of a rat model of type 2 diabetes mellitus. METHODS: The type 2 diabetes mellitus model was developed in diet-induced obesity (DIO) rats followed by the intraperitoneal injection of low-dose streptozotocin (STZ, 25 mg/kg). At four time points during the development of type 2 diabetes mellitus in rats, the fasting serum insulin, leptin, and plasma ghrelin were measured and the hypothalamic neuropeptide Y (NPY) content and mRNA expression were detected in the rats, which were divided into 4 groups: normal control (NC), DIO(4W), DIO(8W), and T2DM; the mRNA expression of OB-Rb, and GSH-R1a in the hypothalamus were also assayed. RESULTS: During the development of the type 2 diabetes mellitus rat model, both the fasting serum levels of insulin and leptin (ng/ml) elevated significantly and the fasting plasma ghrelin concentration decreased the hypothalamic NPY (pg/mg) content significantly. NPY mRNA increased significantly in a time-dependent fashion while both the OB-Rb and the GHS-R1a mRNA of the hypothalamus decreased significantly. Hypothalamic NPY concentration was positively correlated with the changes in serum insulin and leptin and negatively correlated with plasma ghrelin. CONCLUSIONS: During the development of the type 2 diabetes mellitus rat model, the hypothalamic NPY content and NPY mRNA expression increased in a time-dependent manner, which was positively correlated with the changes of the serum insulin and leptin and negatively correlated with the plasma ghrelin. Springer International Publishing 2016-11-03 /pmc/articles/PMC5095108/ /pubmed/27867820 http://dx.doi.org/10.1186/s40064-016-3555-9 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Zhang, Qing-jiu Yang, Chang-chun Zhang, Song-yun Zhang, Li-hui Li, Jie Alteration of NPY in hypothalamus and its correlation with leptin and ghrelin during the development of T2DM in a rat model |
title | Alteration of NPY in hypothalamus and its correlation with leptin and ghrelin during the development of T2DM in a rat model |
title_full | Alteration of NPY in hypothalamus and its correlation with leptin and ghrelin during the development of T2DM in a rat model |
title_fullStr | Alteration of NPY in hypothalamus and its correlation with leptin and ghrelin during the development of T2DM in a rat model |
title_full_unstemmed | Alteration of NPY in hypothalamus and its correlation with leptin and ghrelin during the development of T2DM in a rat model |
title_short | Alteration of NPY in hypothalamus and its correlation with leptin and ghrelin during the development of T2DM in a rat model |
title_sort | alteration of npy in hypothalamus and its correlation with leptin and ghrelin during the development of t2dm in a rat model |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095108/ https://www.ncbi.nlm.nih.gov/pubmed/27867820 http://dx.doi.org/10.1186/s40064-016-3555-9 |
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