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Label-free in situ imaging of oil body dynamics and chemistry in germination

Plant oleosomes are uniquely emulsified lipid reservoirs that serve as the primary energy source during seed germination. These oil bodies undergo significant changes regarding their size, composition and structure during normal seedling development; however, a detailed characterization of these oil...

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Autores principales: Waschatko, Gustav, Billecke, Nils, Schwendy, Sascha, Jaurich, Henriette, Bonn, Mischa, Vilgis, Thomas A., Parekh, Sapun H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095225/
https://www.ncbi.nlm.nih.gov/pubmed/27798279
http://dx.doi.org/10.1098/rsif.2016.0677
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author Waschatko, Gustav
Billecke, Nils
Schwendy, Sascha
Jaurich, Henriette
Bonn, Mischa
Vilgis, Thomas A.
Parekh, Sapun H.
author_facet Waschatko, Gustav
Billecke, Nils
Schwendy, Sascha
Jaurich, Henriette
Bonn, Mischa
Vilgis, Thomas A.
Parekh, Sapun H.
author_sort Waschatko, Gustav
collection PubMed
description Plant oleosomes are uniquely emulsified lipid reservoirs that serve as the primary energy source during seed germination. These oil bodies undergo significant changes regarding their size, composition and structure during normal seedling development; however, a detailed characterization of these oil body dynamics, which critically affect oil body extractability and nutritional value, has remained challenging because of a limited ability to monitor oil body location and composition during germination in situ. Here, we demonstrate via in situ, label-free imaging that oil bodies are highly dynamic intracellular organelles that are morphologically and biochemically remodelled extensively during germination. Label-free, coherent Raman microscopy (CRM) combined with bulk biochemical measurements revealed the temporal and spatial regulation of oil bodies in native soya bean cotyledons during the first eight days of germination. Oil bodies undergo a cycle of growth and shrinkage that is paralleled by lipid and protein compositional changes. Specifically, the total protein concentration associated with oil bodies increases in the first phase of germination and subsequently decreases. Lipids contained within the oil bodies change in saturation and chain length during germination. Our results show that CRM is a well-suited platform to monitor in situ lipid dynamics and local chemistry and that oil bodies are actively remodelled during germination. This underscores the dynamic role of lipid reservoirs in plant development.
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spelling pubmed-50952252016-11-10 Label-free in situ imaging of oil body dynamics and chemistry in germination Waschatko, Gustav Billecke, Nils Schwendy, Sascha Jaurich, Henriette Bonn, Mischa Vilgis, Thomas A. Parekh, Sapun H. J R Soc Interface Life Sciences–Physics interface Plant oleosomes are uniquely emulsified lipid reservoirs that serve as the primary energy source during seed germination. These oil bodies undergo significant changes regarding their size, composition and structure during normal seedling development; however, a detailed characterization of these oil body dynamics, which critically affect oil body extractability and nutritional value, has remained challenging because of a limited ability to monitor oil body location and composition during germination in situ. Here, we demonstrate via in situ, label-free imaging that oil bodies are highly dynamic intracellular organelles that are morphologically and biochemically remodelled extensively during germination. Label-free, coherent Raman microscopy (CRM) combined with bulk biochemical measurements revealed the temporal and spatial regulation of oil bodies in native soya bean cotyledons during the first eight days of germination. Oil bodies undergo a cycle of growth and shrinkage that is paralleled by lipid and protein compositional changes. Specifically, the total protein concentration associated with oil bodies increases in the first phase of germination and subsequently decreases. Lipids contained within the oil bodies change in saturation and chain length during germination. Our results show that CRM is a well-suited platform to monitor in situ lipid dynamics and local chemistry and that oil bodies are actively remodelled during germination. This underscores the dynamic role of lipid reservoirs in plant development. The Royal Society 2016-10 /pmc/articles/PMC5095225/ /pubmed/27798279 http://dx.doi.org/10.1098/rsif.2016.0677 Text en © 2016 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Life Sciences–Physics interface
Waschatko, Gustav
Billecke, Nils
Schwendy, Sascha
Jaurich, Henriette
Bonn, Mischa
Vilgis, Thomas A.
Parekh, Sapun H.
Label-free in situ imaging of oil body dynamics and chemistry in germination
title Label-free in situ imaging of oil body dynamics and chemistry in germination
title_full Label-free in situ imaging of oil body dynamics and chemistry in germination
title_fullStr Label-free in situ imaging of oil body dynamics and chemistry in germination
title_full_unstemmed Label-free in situ imaging of oil body dynamics and chemistry in germination
title_short Label-free in situ imaging of oil body dynamics and chemistry in germination
title_sort label-free in situ imaging of oil body dynamics and chemistry in germination
topic Life Sciences–Physics interface
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095225/
https://www.ncbi.nlm.nih.gov/pubmed/27798279
http://dx.doi.org/10.1098/rsif.2016.0677
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