Cargando…
The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants
β-glucans are well-known modulators of the immune system in mammals but little is known about β-glucan triggered immunity in planta. Here we show by isothermal titration calorimetry, circular dichroism spectroscopy and nuclear magnetic resonance spectroscopy that the FGB1 gene from the root endophyt...
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095285/ https://www.ncbi.nlm.nih.gov/pubmed/27786272 http://dx.doi.org/10.1038/ncomms13188 |
| _version_ | 1782465275805827072 |
|---|---|
| author | Wawra, Stephan Fesel, Philipp Widmer, Heidi Timm, Malte Seibel, Jürgen Leson, Lisa Kesseler, Leona Nostadt, Robin Hilbert, Magdalena Langen, Gregor Zuccaro, Alga |
| author_facet | Wawra, Stephan Fesel, Philipp Widmer, Heidi Timm, Malte Seibel, Jürgen Leson, Lisa Kesseler, Leona Nostadt, Robin Hilbert, Magdalena Langen, Gregor Zuccaro, Alga |
| author_sort | Wawra, Stephan |
| collection | PubMed |
| description | β-glucans are well-known modulators of the immune system in mammals but little is known about β-glucan triggered immunity in planta. Here we show by isothermal titration calorimetry, circular dichroism spectroscopy and nuclear magnetic resonance spectroscopy that the FGB1 gene from the root endophyte Piriformospora indica encodes for a secreted fungal-specific β-glucan-binding lectin with dual function. This lectin has the potential to both alter fungal cell wall composition and properties, and to efficiently suppress β-glucan-triggered immunity in different plant hosts, such as Arabidopsis, barley and Nicotiana benthamiana. Our results hint at the existence of fungal effectors that deregulate innate sensing of β-glucan in plants. |
| format | Online Article Text |
| id | pubmed-5095285 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-50952852016-11-18 The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants Wawra, Stephan Fesel, Philipp Widmer, Heidi Timm, Malte Seibel, Jürgen Leson, Lisa Kesseler, Leona Nostadt, Robin Hilbert, Magdalena Langen, Gregor Zuccaro, Alga Nat Commun Article β-glucans are well-known modulators of the immune system in mammals but little is known about β-glucan triggered immunity in planta. Here we show by isothermal titration calorimetry, circular dichroism spectroscopy and nuclear magnetic resonance spectroscopy that the FGB1 gene from the root endophyte Piriformospora indica encodes for a secreted fungal-specific β-glucan-binding lectin with dual function. This lectin has the potential to both alter fungal cell wall composition and properties, and to efficiently suppress β-glucan-triggered immunity in different plant hosts, such as Arabidopsis, barley and Nicotiana benthamiana. Our results hint at the existence of fungal effectors that deregulate innate sensing of β-glucan in plants. Nature Publishing Group 2016-10-27 /pmc/articles/PMC5095285/ /pubmed/27786272 http://dx.doi.org/10.1038/ncomms13188 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Article Wawra, Stephan Fesel, Philipp Widmer, Heidi Timm, Malte Seibel, Jürgen Leson, Lisa Kesseler, Leona Nostadt, Robin Hilbert, Magdalena Langen, Gregor Zuccaro, Alga The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants |
| title | The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants |
| title_full | The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants |
| title_fullStr | The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants |
| title_full_unstemmed | The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants |
| title_short | The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants |
| title_sort | fungal-specific β-glucan-binding lectin fgb1 alters cell-wall composition and suppresses glucan-triggered immunity in plants |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095285/ https://www.ncbi.nlm.nih.gov/pubmed/27786272 http://dx.doi.org/10.1038/ncomms13188 |
| work_keys_str_mv | AT wawrastephan thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT feselphilipp thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT widmerheidi thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT timmmalte thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT seibeljurgen thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT lesonlisa thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT kesselerleona thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT nostadtrobin thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT hilbertmagdalena thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT langengregor thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT zuccaroalga thefungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT wawrastephan fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT feselphilipp fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT widmerheidi fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT timmmalte fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT seibeljurgen fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT lesonlisa fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT kesselerleona fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT nostadtrobin fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT hilbertmagdalena fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT langengregor fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants AT zuccaroalga fungalspecificbglucanbindinglectinfgb1alterscellwallcompositionandsuppressesglucantriggeredimmunityinplants |