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Mutation rate analysis via parent–progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids

Mutation rates vary between species, between strains within species and between regions within a genome. What are the determinants of these forms of variation? Here, via parent–offspring sequencing of the peach we ask whether (i) woody perennials tend to have lower per unit time mutation rates compa...

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Autores principales: Xie, Zhengqing, Wang, Long, Wang, Lirong, Wang, Zhiqiang, Lu, Zhenhua, Tian, Dacheng, Yang, Sihai, Hurst, Laurence D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095371/
https://www.ncbi.nlm.nih.gov/pubmed/27798292
http://dx.doi.org/10.1098/rspb.2016.1016
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author Xie, Zhengqing
Wang, Long
Wang, Lirong
Wang, Zhiqiang
Lu, Zhenhua
Tian, Dacheng
Yang, Sihai
Hurst, Laurence D.
author_facet Xie, Zhengqing
Wang, Long
Wang, Lirong
Wang, Zhiqiang
Lu, Zhenhua
Tian, Dacheng
Yang, Sihai
Hurst, Laurence D.
author_sort Xie, Zhengqing
collection PubMed
description Mutation rates vary between species, between strains within species and between regions within a genome. What are the determinants of these forms of variation? Here, via parent–offspring sequencing of the peach we ask whether (i) woody perennials tend to have lower per unit time mutation rates compared to annuals, and (ii) hybrid strains have high mutation rates. Between a leaf from a low heterozygosity individual, derived from an intraspecific cross, to a leaf of its selfed progeny, the mutation rate is 7.77 × 10(−9) point mutations per bp per generation, similar to Arabidopsis thaliana (7.0–7.4 × 10(−9) point mutations per bp per generation). This suggests a low per unit time mutation rate as the generation time is much longer in peach. This is supported by our estimate of 9.48 × 10(−9) point mutations per bp per generation from a 200-year-old low heterozygosity peach to its progeny. From a more highly heterozygous individual derived from an interspecific cross to its selfed progeny, the mutation rate is 1.38 × 10(−8) mutations per site per generation, consistent with raised rates in hybrids. Our data thus suggest that (i) peach has an approximately order of magnitude lower mutation rate per unit time than Arabidopsis, consistent with reports of low evolutionary rates in woody perennials, and (ii) hybridization may, indeed, be associated with increased mutation rates as considered over a century ago.
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spelling pubmed-50953712016-11-16 Mutation rate analysis via parent–progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids Xie, Zhengqing Wang, Long Wang, Lirong Wang, Zhiqiang Lu, Zhenhua Tian, Dacheng Yang, Sihai Hurst, Laurence D. Proc Biol Sci Research Articles Mutation rates vary between species, between strains within species and between regions within a genome. What are the determinants of these forms of variation? Here, via parent–offspring sequencing of the peach we ask whether (i) woody perennials tend to have lower per unit time mutation rates compared to annuals, and (ii) hybrid strains have high mutation rates. Between a leaf from a low heterozygosity individual, derived from an intraspecific cross, to a leaf of its selfed progeny, the mutation rate is 7.77 × 10(−9) point mutations per bp per generation, similar to Arabidopsis thaliana (7.0–7.4 × 10(−9) point mutations per bp per generation). This suggests a low per unit time mutation rate as the generation time is much longer in peach. This is supported by our estimate of 9.48 × 10(−9) point mutations per bp per generation from a 200-year-old low heterozygosity peach to its progeny. From a more highly heterozygous individual derived from an interspecific cross to its selfed progeny, the mutation rate is 1.38 × 10(−8) mutations per site per generation, consistent with raised rates in hybrids. Our data thus suggest that (i) peach has an approximately order of magnitude lower mutation rate per unit time than Arabidopsis, consistent with reports of low evolutionary rates in woody perennials, and (ii) hybridization may, indeed, be associated with increased mutation rates as considered over a century ago. The Royal Society 2016-10-26 /pmc/articles/PMC5095371/ /pubmed/27798292 http://dx.doi.org/10.1098/rspb.2016.1016 Text en © 2016 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research Articles
Xie, Zhengqing
Wang, Long
Wang, Lirong
Wang, Zhiqiang
Lu, Zhenhua
Tian, Dacheng
Yang, Sihai
Hurst, Laurence D.
Mutation rate analysis via parent–progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids
title Mutation rate analysis via parent–progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids
title_full Mutation rate analysis via parent–progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids
title_fullStr Mutation rate analysis via parent–progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids
title_full_unstemmed Mutation rate analysis via parent–progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids
title_short Mutation rate analysis via parent–progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids
title_sort mutation rate analysis via parent–progeny sequencing of the perennial peach. i. a low rate in woody perennials and a higher mutagenicity in hybrids
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095371/
https://www.ncbi.nlm.nih.gov/pubmed/27798292
http://dx.doi.org/10.1098/rspb.2016.1016
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