Transmission of atypical scrapie to homozygous ARQ sheep

Two Cheviot ewes homozygous for the A(136)L(141)R(154)Q(171) (AL(141)RQ) prion protein (PrP) genotype were exposed intracerebrally to brain pools prepared using four field cases of atypical scrapie from the United Kingdom. Animals were clinically normal until the end of the experiment, when they wer...

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Detalles Bibliográficos
Autores principales: OKADA, Hiroyuki, MIYAZAWA, Kohtaro, IMAMURA, Morikazu, IWAMARU, Yoshifumi, MASUJIN, Kentaro, MATSUURA, Yuichi, YOKOYAMA, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Pathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095634/
https://www.ncbi.nlm.nih.gov/pubmed/27320968
http://dx.doi.org/10.1292/jvms.16-0259
Descripción
Sumario:Two Cheviot ewes homozygous for the A(136)L(141)R(154)Q(171) (AL(141)RQ) prion protein (PrP) genotype were exposed intracerebrally to brain pools prepared using four field cases of atypical scrapie from the United Kingdom. Animals were clinically normal until the end of the experiment, when they were culled 7 years post-inoculation. Limited accumulation of disease-associated PrP (PrP(Sc)) was observed in the cerebellar molecular layer by immunohistochemistry, but not by western blot or enzyme-linked immunosorbent assay. In addition, PrP(Sc) was partially localized in astrocytes and microglia, suggesting that these cells have a role in PrP(Sc) processing, degradation or both. Our results indicate that atypical scrapie is transmissible to AL(141)RQ sheep, but these animals act as clinically silent carriers with long incubation times.

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