Cargando…

A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers

Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β‐O‐4 linkages. Current thioacidolysis methods are low‐throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis me...

Descripción completa

Detalles Bibliográficos
Autores principales: Harman‐Ware, Anne E., Foster, Cliff, Happs, Renee M., Doeppke, Crissa, Meunier, Kristoffer, Gehan, Jackson, Yue, Fengxia, Lu, Fachuang, Davis, Mark F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY‐VCH Verlag 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096032/
https://www.ncbi.nlm.nih.gov/pubmed/27534715
http://dx.doi.org/10.1002/biot.201600266
_version_ 1782465399033430016
author Harman‐Ware, Anne E.
Foster, Cliff
Happs, Renee M.
Doeppke, Crissa
Meunier, Kristoffer
Gehan, Jackson
Yue, Fengxia
Lu, Fachuang
Davis, Mark F.
author_facet Harman‐Ware, Anne E.
Foster, Cliff
Happs, Renee M.
Doeppke, Crissa
Meunier, Kristoffer
Gehan, Jackson
Yue, Fengxia
Lu, Fachuang
Davis, Mark F.
author_sort Harman‐Ware, Anne E.
collection PubMed
description Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β‐O‐4 linkages. Current thioacidolysis methods are low‐throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non‐chlorinated organic solvent and is tailored for higher‐throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1–2 mg of biomass per assay and has been quantified using fast‐GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day‐to‐day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. The method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.
format Online
Article
Text
id pubmed-5096032
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher WILEY‐VCH Verlag
record_format MEDLINE/PubMed
spelling pubmed-50960322016-11-09 A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers Harman‐Ware, Anne E. Foster, Cliff Happs, Renee M. Doeppke, Crissa Meunier, Kristoffer Gehan, Jackson Yue, Fengxia Lu, Fachuang Davis, Mark F. Biotechnol J Biotech Methods Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β‐O‐4 linkages. Current thioacidolysis methods are low‐throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non‐chlorinated organic solvent and is tailored for higher‐throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1–2 mg of biomass per assay and has been quantified using fast‐GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day‐to‐day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. The method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses. WILEY‐VCH Verlag 2016-09-14 2016-10 /pmc/articles/PMC5096032/ /pubmed/27534715 http://dx.doi.org/10.1002/biot.201600266 Text en © 2016 The Authors. Biotechnology Journal published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biotech Methods
Harman‐Ware, Anne E.
Foster, Cliff
Happs, Renee M.
Doeppke, Crissa
Meunier, Kristoffer
Gehan, Jackson
Yue, Fengxia
Lu, Fachuang
Davis, Mark F.
A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers
title A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers
title_full A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers
title_fullStr A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers
title_full_unstemmed A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers
title_short A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers
title_sort thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers
topic Biotech Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096032/
https://www.ncbi.nlm.nih.gov/pubmed/27534715
http://dx.doi.org/10.1002/biot.201600266
work_keys_str_mv AT harmanwareannee athioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT fostercliff athioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT happsreneem athioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT doeppkecrissa athioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT meunierkristoffer athioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT gehanjackson athioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT yuefengxia athioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT lufachuang athioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT davismarkf athioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT harmanwareannee thioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT fostercliff thioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT happsreneem thioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT doeppkecrissa thioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT meunierkristoffer thioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT gehanjackson thioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT yuefengxia thioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT lufachuang thioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers
AT davismarkf thioacidolysismethodtailoredforhigherthroughputquantitativeanalysisofligninmonomers