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Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages

BACKGROUND: Psoroptic mange is a chronic, refractory, contagious and infectious disease mainly caused by the mange mite Psoroptes ovis, which can infect horses, sheep, buffaloes, rabbits, other domestic animals, deer, wild camels, foxes, minks, lemurs, alpacas, elks and other wild animals. Features...

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Autores principales: He, Man-Li, Xu, Jing, He, Ran, Shen, Neng-Xing, Gu, Xiao-Bin, Peng, Xue-Rong, Yang, Guang-You
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096302/
https://www.ncbi.nlm.nih.gov/pubmed/27809902
http://dx.doi.org/10.1186/s13071-016-1856-z
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author He, Man-Li
Xu, Jing
He, Ran
Shen, Neng-Xing
Gu, Xiao-Bin
Peng, Xue-Rong
Yang, Guang-You
author_facet He, Man-Li
Xu, Jing
He, Ran
Shen, Neng-Xing
Gu, Xiao-Bin
Peng, Xue-Rong
Yang, Guang-You
author_sort He, Man-Li
collection PubMed
description BACKGROUND: Psoroptic mange is a chronic, refractory, contagious and infectious disease mainly caused by the mange mite Psoroptes ovis, which can infect horses, sheep, buffaloes, rabbits, other domestic animals, deer, wild camels, foxes, minks, lemurs, alpacas, elks and other wild animals. Features of the disease include intense pruritus and dermatitis, depilation and hyperkeratosis, which ultimately result in emaciation or death caused by secondary bacterial infections. The infestation is usually transmitted by close contact between animals. Psoroptic mange is widespread in the world. In this paper, the transcriptome of P. ovis is described following sequencing and analysis of transcripts from samples of larvae (i.e. the Pso_L group) and nymphs and adults (i.e. the Pso_N_A group). The study describes differentially expressed genes (DEGs) and genes encoding allergens, which help understanding the biology of P. ovis and lay foundations for the development of vaccine antigens and drug target screening. METHODS: The transcriptome of P. ovis was assembled and analyzed using bioinformatic tools. The unigenes of P. ovis from each developmental stage and the unigenes differentially between developmental stages were compared with allergen protein sequences contained in the allergen database website to predict potential allergens. RESULTS: We identified 38,836 unigenes, whose mean length was 825 bp. On the basis of sequence similarity with seven databases, a total of 17,366 unigenes were annotated. A total of 1,316 DEGs were identified, including 496 upregulated and 820 downregulated in the Pso_L group compared with the Pso_N_A group. We predicted 205 allergens genes in the two developmental stages similar to genes from other mites and ticks, of these, 14 were among the upregulated DEGs and 26 among the downregulated DEGs. CONCLUSION: This study provides a reference transcriptome of P. ovis in absence of a reference genome. The analysis of DEGs and putative allergen genes may lay the foundation for studies of functional genomics, immunity and gene expression profiles of this parasitic mite species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1856-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-50963022016-11-07 Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages He, Man-Li Xu, Jing He, Ran Shen, Neng-Xing Gu, Xiao-Bin Peng, Xue-Rong Yang, Guang-You Parasit Vectors Research BACKGROUND: Psoroptic mange is a chronic, refractory, contagious and infectious disease mainly caused by the mange mite Psoroptes ovis, which can infect horses, sheep, buffaloes, rabbits, other domestic animals, deer, wild camels, foxes, minks, lemurs, alpacas, elks and other wild animals. Features of the disease include intense pruritus and dermatitis, depilation and hyperkeratosis, which ultimately result in emaciation or death caused by secondary bacterial infections. The infestation is usually transmitted by close contact between animals. Psoroptic mange is widespread in the world. In this paper, the transcriptome of P. ovis is described following sequencing and analysis of transcripts from samples of larvae (i.e. the Pso_L group) and nymphs and adults (i.e. the Pso_N_A group). The study describes differentially expressed genes (DEGs) and genes encoding allergens, which help understanding the biology of P. ovis and lay foundations for the development of vaccine antigens and drug target screening. METHODS: The transcriptome of P. ovis was assembled and analyzed using bioinformatic tools. The unigenes of P. ovis from each developmental stage and the unigenes differentially between developmental stages were compared with allergen protein sequences contained in the allergen database website to predict potential allergens. RESULTS: We identified 38,836 unigenes, whose mean length was 825 bp. On the basis of sequence similarity with seven databases, a total of 17,366 unigenes were annotated. A total of 1,316 DEGs were identified, including 496 upregulated and 820 downregulated in the Pso_L group compared with the Pso_N_A group. We predicted 205 allergens genes in the two developmental stages similar to genes from other mites and ticks, of these, 14 were among the upregulated DEGs and 26 among the downregulated DEGs. CONCLUSION: This study provides a reference transcriptome of P. ovis in absence of a reference genome. The analysis of DEGs and putative allergen genes may lay the foundation for studies of functional genomics, immunity and gene expression profiles of this parasitic mite species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1856-z) contains supplementary material, which is available to authorized users. BioMed Central 2016-11-04 /pmc/articles/PMC5096302/ /pubmed/27809902 http://dx.doi.org/10.1186/s13071-016-1856-z Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
He, Man-Li
Xu, Jing
He, Ran
Shen, Neng-Xing
Gu, Xiao-Bin
Peng, Xue-Rong
Yang, Guang-You
Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages
title Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages
title_full Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages
title_fullStr Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages
title_full_unstemmed Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages
title_short Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages
title_sort preliminary analysis of psoroptes ovis transcriptome in different developmental stages
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096302/
https://www.ncbi.nlm.nih.gov/pubmed/27809902
http://dx.doi.org/10.1186/s13071-016-1856-z
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