Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease

The type II CRISPR-associated protein Cas9 recognizes and cleaves target DNA with the help of two guide RNAs (gRNAs; tracrRNA and crRNA). However, the detailed mechanisms and kinetics of these gRNAs in the Cas9 nuclease activity are unclear. Here, we investigate the structural roles of gRNAs in the...

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Detalles Bibliográficos
Autores principales: Lim, Youngbin, Bak, So Young, Sung, Keewon, Jeong, Euihwan, Lee, Seung Hwan, Kim, Jin-Soo, Bae, Sangsu, Kim, Seong Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5097132/
https://www.ncbi.nlm.nih.gov/pubmed/27804953
http://dx.doi.org/10.1038/ncomms13350

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5097132/
https://www.ncbi.nlm.nih.gov/pubmed/27804953
http://dx.doi.org/10.1038/ncomms13350

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