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Quality filtering of Illumina index reads mitigates sample cross-talk

BACKGROUND: Multiplexing multiple samples during Illumina sequencing is a common practice and is rapidly growing in importance as the throughput of the platform increases. Misassignments during de-multiplexing, where sequences are associated with the wrong sample, are an overlooked error mode on the...

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Detalles Bibliográficos
Autores principales: Wright, Erik Scott, Vetsigian, Kalin Horen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5097354/
https://www.ncbi.nlm.nih.gov/pubmed/27814679
http://dx.doi.org/10.1186/s12864-016-3217-x