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Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq

BACKGROUND: The robustness of ChIP-seq datasets is highly dependent upon the antibodies used. Currently, polyclonal antibodies are the standard despite several limitations: They are non-renewable, vary in performance between lots and need to be validated with each new lot. In contrast, monoclonal an...

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Detalles Bibliográficos
Autores principales:	Busby, Michele, Xue, Catherine, Li, Catherine, Farjoun, Yossi, Gienger, Elizabeth, Yofe, Ido, Gladden, Adrianne, Epstein, Charles B., Cornett, Evan M., Rothbart, Scott B., Nusbaum, Chad, Goren, Alon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5097419/ https://www.ncbi.nlm.nih.gov/pubmed/27826357 http://dx.doi.org/10.1186/s13072-016-0100-6

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