A rapid, automated surface protein profiling of single circulating exosomes in human blood

Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due to their stability, accessibility and representation of molecules from source cells. However, this potential has been stymied by lack of approaches for molecular profiling of individual exo...

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Autores principales: Kibria, Golam, Ramos, Erika K., Lee, Katelyn E., Bedoyan, Sarah, Huang, Simo, Samaeekia, Ravand, Athman, Jaffre J., Harding, Clifford V., Lötvall, Jan, Harris, Lyndsay, Thompson, Cheryl L., Liu, Huiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098148/
https://www.ncbi.nlm.nih.gov/pubmed/27819324
http://dx.doi.org/10.1038/srep36502
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author Kibria, Golam
Ramos, Erika K.
Lee, Katelyn E.
Bedoyan, Sarah
Huang, Simo
Samaeekia, Ravand
Athman, Jaffre J.
Harding, Clifford V.
Lötvall, Jan
Harris, Lyndsay
Thompson, Cheryl L.
Liu, Huiping
author_facet Kibria, Golam
Ramos, Erika K.
Lee, Katelyn E.
Bedoyan, Sarah
Huang, Simo
Samaeekia, Ravand
Athman, Jaffre J.
Harding, Clifford V.
Lötvall, Jan
Harris, Lyndsay
Thompson, Cheryl L.
Liu, Huiping
author_sort Kibria, Golam
collection PubMed
description Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due to their stability, accessibility and representation of molecules from source cells. However, this potential has been stymied by lack of approaches for molecular profiling of individual exosomes, which have a diameter of 30–150 nm. Here we report a rapid analysis approach to evaluate heterogeneous surface protein expression in single circulating exosomes from human blood. Our studies show a differential CD47 expression in blood-derived individual circulating exosomes that is correlated with breast cancer status, demonstrating a great potential of individual exosome profiles in biomarker discovery. The sensitive and high throughput platform of single exosome analysis can also be applied to characterizing exosomes derived from other patient fluids.
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spelling pubmed-50981482016-11-10 A rapid, automated surface protein profiling of single circulating exosomes in human blood Kibria, Golam Ramos, Erika K. Lee, Katelyn E. Bedoyan, Sarah Huang, Simo Samaeekia, Ravand Athman, Jaffre J. Harding, Clifford V. Lötvall, Jan Harris, Lyndsay Thompson, Cheryl L. Liu, Huiping Sci Rep Article Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due to their stability, accessibility and representation of molecules from source cells. However, this potential has been stymied by lack of approaches for molecular profiling of individual exosomes, which have a diameter of 30–150 nm. Here we report a rapid analysis approach to evaluate heterogeneous surface protein expression in single circulating exosomes from human blood. Our studies show a differential CD47 expression in blood-derived individual circulating exosomes that is correlated with breast cancer status, demonstrating a great potential of individual exosome profiles in biomarker discovery. The sensitive and high throughput platform of single exosome analysis can also be applied to characterizing exosomes derived from other patient fluids. Nature Publishing Group 2016-11-07 /pmc/articles/PMC5098148/ /pubmed/27819324 http://dx.doi.org/10.1038/srep36502 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kibria, Golam
Ramos, Erika K.
Lee, Katelyn E.
Bedoyan, Sarah
Huang, Simo
Samaeekia, Ravand
Athman, Jaffre J.
Harding, Clifford V.
Lötvall, Jan
Harris, Lyndsay
Thompson, Cheryl L.
Liu, Huiping
A rapid, automated surface protein profiling of single circulating exosomes in human blood
title A rapid, automated surface protein profiling of single circulating exosomes in human blood
title_full A rapid, automated surface protein profiling of single circulating exosomes in human blood
title_fullStr A rapid, automated surface protein profiling of single circulating exosomes in human blood
title_full_unstemmed A rapid, automated surface protein profiling of single circulating exosomes in human blood
title_short A rapid, automated surface protein profiling of single circulating exosomes in human blood
title_sort rapid, automated surface protein profiling of single circulating exosomes in human blood
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098148/
https://www.ncbi.nlm.nih.gov/pubmed/27819324
http://dx.doi.org/10.1038/srep36502
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