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Serum neurofilament light protein predicts clinical outcome in traumatic brain injury
Axonal white matter injury is believed to be a major determinant of adverse outcomes following traumatic brain injury (TBI). We hypothesized that measurement of neurofilament light protein (NF-L), a protein found in long white-matter axons, in blood samples, may serve as a suitable biomarker for neu...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098187/ https://www.ncbi.nlm.nih.gov/pubmed/27819296 http://dx.doi.org/10.1038/srep36791 |
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author | Shahim, Pashtun Gren, Magnus Liman, Victor Andreasson, Ulf Norgren, Niklas Tegner, Yelverton Mattsson, Niklas Andreasen, Niels Öst, Martin Zetterberg, Henrik Nellgård, Bengt Blennow, Kaj |
author_facet | Shahim, Pashtun Gren, Magnus Liman, Victor Andreasson, Ulf Norgren, Niklas Tegner, Yelverton Mattsson, Niklas Andreasen, Niels Öst, Martin Zetterberg, Henrik Nellgård, Bengt Blennow, Kaj |
author_sort | Shahim, Pashtun |
collection | PubMed |
description | Axonal white matter injury is believed to be a major determinant of adverse outcomes following traumatic brain injury (TBI). We hypothesized that measurement of neurofilament light protein (NF-L), a protein found in long white-matter axons, in blood samples, may serve as a suitable biomarker for neuronal damage in TBI patients. To test our hypotheses, we designed a study in two parts: i) we developed an immunoassay based on Single molecule array technology for quantification of NF-L in blood, and ii) in a proof-of-concept study, we tested our newly developed method on serial serum samples from severe TBI (sTBI) patients (n = 72) and controls (n = 35). We also compared the diagnostic and prognostic utility of NF-L with the established blood biomarker S100B. NF-L levels were markedly increased in sTBI patients compared with controls. NF-L at admission yielded an AUC of 0.99 to detect TBI versus controls (AUC 0.96 for S100B), and increased to 1.00 at day 12 (0.65 for S100B). Importantly, initial NF-L levels predicted poor 12-month clinical outcome. In contrast, S100B was not related to outcome. Taken together, our data suggests that measurement of serum NF-L may be useful to assess the severity of neuronal injury following sTBI. |
format | Online Article Text |
id | pubmed-5098187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50981872016-11-10 Serum neurofilament light protein predicts clinical outcome in traumatic brain injury Shahim, Pashtun Gren, Magnus Liman, Victor Andreasson, Ulf Norgren, Niklas Tegner, Yelverton Mattsson, Niklas Andreasen, Niels Öst, Martin Zetterberg, Henrik Nellgård, Bengt Blennow, Kaj Sci Rep Article Axonal white matter injury is believed to be a major determinant of adverse outcomes following traumatic brain injury (TBI). We hypothesized that measurement of neurofilament light protein (NF-L), a protein found in long white-matter axons, in blood samples, may serve as a suitable biomarker for neuronal damage in TBI patients. To test our hypotheses, we designed a study in two parts: i) we developed an immunoassay based on Single molecule array technology for quantification of NF-L in blood, and ii) in a proof-of-concept study, we tested our newly developed method on serial serum samples from severe TBI (sTBI) patients (n = 72) and controls (n = 35). We also compared the diagnostic and prognostic utility of NF-L with the established blood biomarker S100B. NF-L levels were markedly increased in sTBI patients compared with controls. NF-L at admission yielded an AUC of 0.99 to detect TBI versus controls (AUC 0.96 for S100B), and increased to 1.00 at day 12 (0.65 for S100B). Importantly, initial NF-L levels predicted poor 12-month clinical outcome. In contrast, S100B was not related to outcome. Taken together, our data suggests that measurement of serum NF-L may be useful to assess the severity of neuronal injury following sTBI. Nature Publishing Group 2016-11-07 /pmc/articles/PMC5098187/ /pubmed/27819296 http://dx.doi.org/10.1038/srep36791 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Shahim, Pashtun Gren, Magnus Liman, Victor Andreasson, Ulf Norgren, Niklas Tegner, Yelverton Mattsson, Niklas Andreasen, Niels Öst, Martin Zetterberg, Henrik Nellgård, Bengt Blennow, Kaj Serum neurofilament light protein predicts clinical outcome in traumatic brain injury |
title | Serum neurofilament light protein predicts clinical outcome in traumatic brain injury |
title_full | Serum neurofilament light protein predicts clinical outcome in traumatic brain injury |
title_fullStr | Serum neurofilament light protein predicts clinical outcome in traumatic brain injury |
title_full_unstemmed | Serum neurofilament light protein predicts clinical outcome in traumatic brain injury |
title_short | Serum neurofilament light protein predicts clinical outcome in traumatic brain injury |
title_sort | serum neurofilament light protein predicts clinical outcome in traumatic brain injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098187/ https://www.ncbi.nlm.nih.gov/pubmed/27819296 http://dx.doi.org/10.1038/srep36791 |
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