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Antibacterial abilities and biocompatibilities of Ti–Ag alloys with nanotubular coatings
PURPOSE: To endow implants with both short- and long-term antibacterial activities without impairing their biocompatibility, novel Ti–Ag alloy substrates with different proportions of Ag (1, 2, and 4 wt% Ag) were generated with nanotubular coverings (TiAg-NT). METHODS: Unlike commercial pure Ti and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098752/ https://www.ncbi.nlm.nih.gov/pubmed/27843315 http://dx.doi.org/10.2147/IJN.S113674 |
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author | Liu, Xingwang Tian, Ang You, Junhua Zhang, Hangzhou Wu, Lin Bai, Xizhuang Lei, Zeming Shi, Xiaoguo Xue, Xiangxin Wang, Hanning |
author_facet | Liu, Xingwang Tian, Ang You, Junhua Zhang, Hangzhou Wu, Lin Bai, Xizhuang Lei, Zeming Shi, Xiaoguo Xue, Xiangxin Wang, Hanning |
author_sort | Liu, Xingwang |
collection | PubMed |
description | PURPOSE: To endow implants with both short- and long-term antibacterial activities without impairing their biocompatibility, novel Ti–Ag alloy substrates with different proportions of Ag (1, 2, and 4 wt% Ag) were generated with nanotubular coverings (TiAg-NT). METHODS: Unlike commercial pure Ti and titania nanotube, the TiAg-NT samples exhibited short-term antibacterial activity against Staphylococcus aureus (S. aureus), as confirmed by scanning electron microscopy and double staining with SYTO 9 and propidium iodide. A film applicator coating assay and a zone of inhibition assay were performed to investigate the long-term antibacterial activities of the samples. The cellular viability and cytotoxicity were evaluated through a Cell Counting Kit-8 assay. Annexin V-FITC/propidium iodide double staining was used to assess the level of MG63 cell apoptosis on each sample. RESULTS: All of the TiAg-NT samples, particularly the nanotube-coated Ti–Ag alloy with 2 wt% Ag (Ti2%Ag-NT), could effectively inhibit bacterial adhesion and kill the majority of adhered S. aureus on the first day of culture. Additionally, the excellent antibacterial abilities exhibited by the TiAg-NT samples were sustained for at least 30 days. Although Ti2%Ag-NT had less biocompatibility than titania nanotube, its performance was satisfactory, as demonstrated by the higher cellular viability and lower cell apoptosis rate obtained with it compared with those achieved with commercial pure Ti. The Ti1%Ag-NT and Ti4%Ag-NT samples did not yield good cell viability. CONCLUSION: This study indicates that the TiAg-NT samples can prevent biofilm formation and maintain their antibacterial ability for at least 1 month. Ti2%Ag-NT exhibited better antibacterial ability and biocompatibility than commercial pure Ti, which could be attributed to the synergistic effect of the presence of Ag (2 wt%) and the morphology of the nanotubes. Ti2%Ag-NT may offer a potential implant material that is capable of preventing implant-related infection. |
format | Online Article Text |
id | pubmed-5098752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-50987522016-11-14 Antibacterial abilities and biocompatibilities of Ti–Ag alloys with nanotubular coatings Liu, Xingwang Tian, Ang You, Junhua Zhang, Hangzhou Wu, Lin Bai, Xizhuang Lei, Zeming Shi, Xiaoguo Xue, Xiangxin Wang, Hanning Int J Nanomedicine Original Research PURPOSE: To endow implants with both short- and long-term antibacterial activities without impairing their biocompatibility, novel Ti–Ag alloy substrates with different proportions of Ag (1, 2, and 4 wt% Ag) were generated with nanotubular coverings (TiAg-NT). METHODS: Unlike commercial pure Ti and titania nanotube, the TiAg-NT samples exhibited short-term antibacterial activity against Staphylococcus aureus (S. aureus), as confirmed by scanning electron microscopy and double staining with SYTO 9 and propidium iodide. A film applicator coating assay and a zone of inhibition assay were performed to investigate the long-term antibacterial activities of the samples. The cellular viability and cytotoxicity were evaluated through a Cell Counting Kit-8 assay. Annexin V-FITC/propidium iodide double staining was used to assess the level of MG63 cell apoptosis on each sample. RESULTS: All of the TiAg-NT samples, particularly the nanotube-coated Ti–Ag alloy with 2 wt% Ag (Ti2%Ag-NT), could effectively inhibit bacterial adhesion and kill the majority of adhered S. aureus on the first day of culture. Additionally, the excellent antibacterial abilities exhibited by the TiAg-NT samples were sustained for at least 30 days. Although Ti2%Ag-NT had less biocompatibility than titania nanotube, its performance was satisfactory, as demonstrated by the higher cellular viability and lower cell apoptosis rate obtained with it compared with those achieved with commercial pure Ti. The Ti1%Ag-NT and Ti4%Ag-NT samples did not yield good cell viability. CONCLUSION: This study indicates that the TiAg-NT samples can prevent biofilm formation and maintain their antibacterial ability for at least 1 month. Ti2%Ag-NT exhibited better antibacterial ability and biocompatibility than commercial pure Ti, which could be attributed to the synergistic effect of the presence of Ag (2 wt%) and the morphology of the nanotubes. Ti2%Ag-NT may offer a potential implant material that is capable of preventing implant-related infection. Dove Medical Press 2016-11-02 /pmc/articles/PMC5098752/ /pubmed/27843315 http://dx.doi.org/10.2147/IJN.S113674 Text en © 2016 Liu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Liu, Xingwang Tian, Ang You, Junhua Zhang, Hangzhou Wu, Lin Bai, Xizhuang Lei, Zeming Shi, Xiaoguo Xue, Xiangxin Wang, Hanning Antibacterial abilities and biocompatibilities of Ti–Ag alloys with nanotubular coatings |
title | Antibacterial abilities and biocompatibilities of Ti–Ag alloys with nanotubular coatings |
title_full | Antibacterial abilities and biocompatibilities of Ti–Ag alloys with nanotubular coatings |
title_fullStr | Antibacterial abilities and biocompatibilities of Ti–Ag alloys with nanotubular coatings |
title_full_unstemmed | Antibacterial abilities and biocompatibilities of Ti–Ag alloys with nanotubular coatings |
title_short | Antibacterial abilities and biocompatibilities of Ti–Ag alloys with nanotubular coatings |
title_sort | antibacterial abilities and biocompatibilities of ti–ag alloys with nanotubular coatings |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098752/ https://www.ncbi.nlm.nih.gov/pubmed/27843315 http://dx.doi.org/10.2147/IJN.S113674 |
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