A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice

Stroke is one of the common causes of death and disability in the world. The intraluminal middle cerebral artery occlusion/reperfusion (MCAO/R) model is a “gold standard” in surgical ischemic stroke models. Here, we optimized the procedure of this model by ligating on external carotid artery (ECA) s...

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Autores principales: Cai, Qiang, Xu, Gang, Liu, Junhui, Wang, Long, Deng, Gang, Liu, Jun, Chen, Zhibiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098775/
https://www.ncbi.nlm.nih.gov/pubmed/27843320
http://dx.doi.org/10.2147/NDT.S118531
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author Cai, Qiang
Xu, Gang
Liu, Junhui
Wang, Long
Deng, Gang
Liu, Jun
Chen, Zhibiao
author_facet Cai, Qiang
Xu, Gang
Liu, Junhui
Wang, Long
Deng, Gang
Liu, Jun
Chen, Zhibiao
author_sort Cai, Qiang
collection PubMed
description Stroke is one of the common causes of death and disability in the world. The intraluminal middle cerebral artery occlusion/reperfusion (MCAO/R) model is a “gold standard” in surgical ischemic stroke models. Here, we optimized the procedure of this model by ligating on external carotid artery (ECA) stump and two ligatures prepared on internal carotid artery, which could improve the success and survival rate in mice. The results show that ECA approach was superior to common carotid artery approach. Meanwhile, we found that the exposure of pterygopalatine artery was not an essential step for MCAO/R model in mice.
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spelling pubmed-50987752016-11-14 A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice Cai, Qiang Xu, Gang Liu, Junhui Wang, Long Deng, Gang Liu, Jun Chen, Zhibiao Neuropsychiatr Dis Treat Methodology Stroke is one of the common causes of death and disability in the world. The intraluminal middle cerebral artery occlusion/reperfusion (MCAO/R) model is a “gold standard” in surgical ischemic stroke models. Here, we optimized the procedure of this model by ligating on external carotid artery (ECA) stump and two ligatures prepared on internal carotid artery, which could improve the success and survival rate in mice. The results show that ECA approach was superior to common carotid artery approach. Meanwhile, we found that the exposure of pterygopalatine artery was not an essential step for MCAO/R model in mice. Dove Medical Press 2016-11-03 /pmc/articles/PMC5098775/ /pubmed/27843320 http://dx.doi.org/10.2147/NDT.S118531 Text en © 2016 Cai et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Methodology
Cai, Qiang
Xu, Gang
Liu, Junhui
Wang, Long
Deng, Gang
Liu, Jun
Chen, Zhibiao
A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice
title A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice
title_full A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice
title_fullStr A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice
title_full_unstemmed A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice
title_short A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice
title_sort modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser doppler flowmetry guidance in mice
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098775/
https://www.ncbi.nlm.nih.gov/pubmed/27843320
http://dx.doi.org/10.2147/NDT.S118531
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