Cargando…

Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates

Candida tropicalis is an important pathogen. Here we developed and evaluated a polymorphic multilocus microsatellite scheme employing novel genetic markers for genotyping of C. tropicalis. Using 10 isolates from 10 unique (separate) patients to screen over 4000 tandem repeats from the C. tropicalis...

Descripción completa

Detalles Bibliográficos
Autores principales: Fan, Xin, Xiao, Meng, Liu, Ping, Chen, Sharon, Kong, Fanrong, Wang, He, Zhang, Li, Hou, Xin, Xu, Ying-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098789/
https://www.ncbi.nlm.nih.gov/pubmed/27820850
http://dx.doi.org/10.1371/journal.pone.0166156
_version_ 1782465829213831168
author Fan, Xin
Xiao, Meng
Liu, Ping
Chen, Sharon
Kong, Fanrong
Wang, He
Zhang, Li
Hou, Xin
Xu, Ying-Chun
author_facet Fan, Xin
Xiao, Meng
Liu, Ping
Chen, Sharon
Kong, Fanrong
Wang, He
Zhang, Li
Hou, Xin
Xu, Ying-Chun
author_sort Fan, Xin
collection PubMed
description Candida tropicalis is an important pathogen. Here we developed and evaluated a polymorphic multilocus microsatellite scheme employing novel genetic markers for genotyping of C. tropicalis. Using 10 isolates from 10 unique (separate) patients to screen over 4000 tandem repeats from the C. tropicalis genome (strain MYA-3404), six new candidate microsatellite loci (ctm1, ctm3, ctm8, ctm18, ctm24 and ctm26) were selected according to amplification success, observed polymorphisms and stability of flanking regions by preliminary testing. Two known microsatellite loci CT14 and URA3 were also studied. The 6-locus scheme was then tested against a set of 82 different isolates from 32 patients. Microsatellite genotypes of isolates from the same patient (two to five isolates per patient) were identical. The six loci produced eight to 17 allele types and identified 11 to 24 genotypes amongst 32 patients’ isolates, achieving a discriminatory power (DP) of 0.76 to 0.97 (versus 0.78 for both CT14 and URA3 loci, respectively). Testing of a combination of only three loci, ctm1, ctm3 and ctm24, also achieved maximum typing efficiency (DP = 0.99, 29 genotypes). The microsatellite typing scheme had good correlation compared with pulsed-field gel electrophoresis, although was slightly less discriminatory. The new six-locus microsatellite typing scheme is a potentially valuable tool for genotyping and investigating microevolution of C. tropicalis.
format Online
Article
Text
id pubmed-5098789
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-50987892016-11-15 Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates Fan, Xin Xiao, Meng Liu, Ping Chen, Sharon Kong, Fanrong Wang, He Zhang, Li Hou, Xin Xu, Ying-Chun PLoS One Research Article Candida tropicalis is an important pathogen. Here we developed and evaluated a polymorphic multilocus microsatellite scheme employing novel genetic markers for genotyping of C. tropicalis. Using 10 isolates from 10 unique (separate) patients to screen over 4000 tandem repeats from the C. tropicalis genome (strain MYA-3404), six new candidate microsatellite loci (ctm1, ctm3, ctm8, ctm18, ctm24 and ctm26) were selected according to amplification success, observed polymorphisms and stability of flanking regions by preliminary testing. Two known microsatellite loci CT14 and URA3 were also studied. The 6-locus scheme was then tested against a set of 82 different isolates from 32 patients. Microsatellite genotypes of isolates from the same patient (two to five isolates per patient) were identical. The six loci produced eight to 17 allele types and identified 11 to 24 genotypes amongst 32 patients’ isolates, achieving a discriminatory power (DP) of 0.76 to 0.97 (versus 0.78 for both CT14 and URA3 loci, respectively). Testing of a combination of only three loci, ctm1, ctm3 and ctm24, also achieved maximum typing efficiency (DP = 0.99, 29 genotypes). The microsatellite typing scheme had good correlation compared with pulsed-field gel electrophoresis, although was slightly less discriminatory. The new six-locus microsatellite typing scheme is a potentially valuable tool for genotyping and investigating microevolution of C. tropicalis. Public Library of Science 2016-11-07 /pmc/articles/PMC5098789/ /pubmed/27820850 http://dx.doi.org/10.1371/journal.pone.0166156 Text en © 2016 Fan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fan, Xin
Xiao, Meng
Liu, Ping
Chen, Sharon
Kong, Fanrong
Wang, He
Zhang, Li
Hou, Xin
Xu, Ying-Chun
Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates
title Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates
title_full Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates
title_fullStr Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates
title_full_unstemmed Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates
title_short Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates
title_sort novel polymorphic multilocus microsatellite markers to distinguish candida tropicalis isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098789/
https://www.ncbi.nlm.nih.gov/pubmed/27820850
http://dx.doi.org/10.1371/journal.pone.0166156
work_keys_str_mv AT fanxin novelpolymorphicmultilocusmicrosatellitemarkerstodistinguishcandidatropicalisisolates
AT xiaomeng novelpolymorphicmultilocusmicrosatellitemarkerstodistinguishcandidatropicalisisolates
AT liuping novelpolymorphicmultilocusmicrosatellitemarkerstodistinguishcandidatropicalisisolates
AT chensharon novelpolymorphicmultilocusmicrosatellitemarkerstodistinguishcandidatropicalisisolates
AT kongfanrong novelpolymorphicmultilocusmicrosatellitemarkerstodistinguishcandidatropicalisisolates
AT wanghe novelpolymorphicmultilocusmicrosatellitemarkerstodistinguishcandidatropicalisisolates
AT zhangli novelpolymorphicmultilocusmicrosatellitemarkerstodistinguishcandidatropicalisisolates
AT houxin novelpolymorphicmultilocusmicrosatellitemarkerstodistinguishcandidatropicalisisolates
AT xuyingchun novelpolymorphicmultilocusmicrosatellitemarkerstodistinguishcandidatropicalisisolates