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Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates
Candida tropicalis is an important pathogen. Here we developed and evaluated a polymorphic multilocus microsatellite scheme employing novel genetic markers for genotyping of C. tropicalis. Using 10 isolates from 10 unique (separate) patients to screen over 4000 tandem repeats from the C. tropicalis...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098789/ https://www.ncbi.nlm.nih.gov/pubmed/27820850 http://dx.doi.org/10.1371/journal.pone.0166156 |
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author | Fan, Xin Xiao, Meng Liu, Ping Chen, Sharon Kong, Fanrong Wang, He Zhang, Li Hou, Xin Xu, Ying-Chun |
author_facet | Fan, Xin Xiao, Meng Liu, Ping Chen, Sharon Kong, Fanrong Wang, He Zhang, Li Hou, Xin Xu, Ying-Chun |
author_sort | Fan, Xin |
collection | PubMed |
description | Candida tropicalis is an important pathogen. Here we developed and evaluated a polymorphic multilocus microsatellite scheme employing novel genetic markers for genotyping of C. tropicalis. Using 10 isolates from 10 unique (separate) patients to screen over 4000 tandem repeats from the C. tropicalis genome (strain MYA-3404), six new candidate microsatellite loci (ctm1, ctm3, ctm8, ctm18, ctm24 and ctm26) were selected according to amplification success, observed polymorphisms and stability of flanking regions by preliminary testing. Two known microsatellite loci CT14 and URA3 were also studied. The 6-locus scheme was then tested against a set of 82 different isolates from 32 patients. Microsatellite genotypes of isolates from the same patient (two to five isolates per patient) were identical. The six loci produced eight to 17 allele types and identified 11 to 24 genotypes amongst 32 patients’ isolates, achieving a discriminatory power (DP) of 0.76 to 0.97 (versus 0.78 for both CT14 and URA3 loci, respectively). Testing of a combination of only three loci, ctm1, ctm3 and ctm24, also achieved maximum typing efficiency (DP = 0.99, 29 genotypes). The microsatellite typing scheme had good correlation compared with pulsed-field gel electrophoresis, although was slightly less discriminatory. The new six-locus microsatellite typing scheme is a potentially valuable tool for genotyping and investigating microevolution of C. tropicalis. |
format | Online Article Text |
id | pubmed-5098789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50987892016-11-15 Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates Fan, Xin Xiao, Meng Liu, Ping Chen, Sharon Kong, Fanrong Wang, He Zhang, Li Hou, Xin Xu, Ying-Chun PLoS One Research Article Candida tropicalis is an important pathogen. Here we developed and evaluated a polymorphic multilocus microsatellite scheme employing novel genetic markers for genotyping of C. tropicalis. Using 10 isolates from 10 unique (separate) patients to screen over 4000 tandem repeats from the C. tropicalis genome (strain MYA-3404), six new candidate microsatellite loci (ctm1, ctm3, ctm8, ctm18, ctm24 and ctm26) were selected according to amplification success, observed polymorphisms and stability of flanking regions by preliminary testing. Two known microsatellite loci CT14 and URA3 were also studied. The 6-locus scheme was then tested against a set of 82 different isolates from 32 patients. Microsatellite genotypes of isolates from the same patient (two to five isolates per patient) were identical. The six loci produced eight to 17 allele types and identified 11 to 24 genotypes amongst 32 patients’ isolates, achieving a discriminatory power (DP) of 0.76 to 0.97 (versus 0.78 for both CT14 and URA3 loci, respectively). Testing of a combination of only three loci, ctm1, ctm3 and ctm24, also achieved maximum typing efficiency (DP = 0.99, 29 genotypes). The microsatellite typing scheme had good correlation compared with pulsed-field gel electrophoresis, although was slightly less discriminatory. The new six-locus microsatellite typing scheme is a potentially valuable tool for genotyping and investigating microevolution of C. tropicalis. Public Library of Science 2016-11-07 /pmc/articles/PMC5098789/ /pubmed/27820850 http://dx.doi.org/10.1371/journal.pone.0166156 Text en © 2016 Fan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Fan, Xin Xiao, Meng Liu, Ping Chen, Sharon Kong, Fanrong Wang, He Zhang, Li Hou, Xin Xu, Ying-Chun Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates |
title | Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates |
title_full | Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates |
title_fullStr | Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates |
title_full_unstemmed | Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates |
title_short | Novel Polymorphic Multilocus Microsatellite Markers to Distinguish Candida tropicalis Isolates |
title_sort | novel polymorphic multilocus microsatellite markers to distinguish candida tropicalis isolates |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098789/ https://www.ncbi.nlm.nih.gov/pubmed/27820850 http://dx.doi.org/10.1371/journal.pone.0166156 |
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