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Zebrafish response to a robotic replica in three dimensions
As zebrafish emerge as a species of choice for the investigation of biological processes, a number of experimental protocols are being developed to study their social behaviour. While live stimuli may elicit varying response in focal subjects owing to idiosyncrasies, tiredness and circadian rhythms,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098991/ https://www.ncbi.nlm.nih.gov/pubmed/27853566 http://dx.doi.org/10.1098/rsos.160505 |
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author | Ruberto, Tommaso Mwaffo, Violet Singh, Sukhgewanpreet Neri, Daniele Porfiri, Maurizio |
author_facet | Ruberto, Tommaso Mwaffo, Violet Singh, Sukhgewanpreet Neri, Daniele Porfiri, Maurizio |
author_sort | Ruberto, Tommaso |
collection | PubMed |
description | As zebrafish emerge as a species of choice for the investigation of biological processes, a number of experimental protocols are being developed to study their social behaviour. While live stimuli may elicit varying response in focal subjects owing to idiosyncrasies, tiredness and circadian rhythms, video stimuli suffer from the absence of physical input and rely only on two-dimensional projections. Robotics has been recently proposed as an alternative approach to generate physical, customizable, effective and consistent stimuli for behavioural phenotyping. Here, we contribute to this field of investigation through a novel four-degree-of-freedom robotics-based platform to manoeuvre a biologically inspired three-dimensionally printed replica. The platform enables three-dimensional motions as well as body oscillations to mimic zebrafish locomotion. In a series of experiments, we demonstrate the differential role of the visual stimuli associated with the biologically inspired replica and its three-dimensional motion. Three-dimensional tracking and information-theoretic tools are complemented to quantify the interaction between zebrafish and the robotic stimulus. Live subjects displayed a robust attraction towards the moving replica, and such attraction was lost when controlling for its visual appearance or motion. This effort is expected to aid zebrafish behavioural phenotyping, by offering a novel approach to generate physical stimuli moving in three dimensions. |
format | Online Article Text |
id | pubmed-5098991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-50989912016-11-16 Zebrafish response to a robotic replica in three dimensions Ruberto, Tommaso Mwaffo, Violet Singh, Sukhgewanpreet Neri, Daniele Porfiri, Maurizio R Soc Open Sci Biology (Whole Organism) As zebrafish emerge as a species of choice for the investigation of biological processes, a number of experimental protocols are being developed to study their social behaviour. While live stimuli may elicit varying response in focal subjects owing to idiosyncrasies, tiredness and circadian rhythms, video stimuli suffer from the absence of physical input and rely only on two-dimensional projections. Robotics has been recently proposed as an alternative approach to generate physical, customizable, effective and consistent stimuli for behavioural phenotyping. Here, we contribute to this field of investigation through a novel four-degree-of-freedom robotics-based platform to manoeuvre a biologically inspired three-dimensionally printed replica. The platform enables three-dimensional motions as well as body oscillations to mimic zebrafish locomotion. In a series of experiments, we demonstrate the differential role of the visual stimuli associated with the biologically inspired replica and its three-dimensional motion. Three-dimensional tracking and information-theoretic tools are complemented to quantify the interaction between zebrafish and the robotic stimulus. Live subjects displayed a robust attraction towards the moving replica, and such attraction was lost when controlling for its visual appearance or motion. This effort is expected to aid zebrafish behavioural phenotyping, by offering a novel approach to generate physical stimuli moving in three dimensions. The Royal Society 2016-10-19 /pmc/articles/PMC5098991/ /pubmed/27853566 http://dx.doi.org/10.1098/rsos.160505 Text en © 2016 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Biology (Whole Organism) Ruberto, Tommaso Mwaffo, Violet Singh, Sukhgewanpreet Neri, Daniele Porfiri, Maurizio Zebrafish response to a robotic replica in three dimensions |
title | Zebrafish response to a robotic replica in three dimensions |
title_full | Zebrafish response to a robotic replica in three dimensions |
title_fullStr | Zebrafish response to a robotic replica in three dimensions |
title_full_unstemmed | Zebrafish response to a robotic replica in three dimensions |
title_short | Zebrafish response to a robotic replica in three dimensions |
title_sort | zebrafish response to a robotic replica in three dimensions |
topic | Biology (Whole Organism) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5098991/ https://www.ncbi.nlm.nih.gov/pubmed/27853566 http://dx.doi.org/10.1098/rsos.160505 |
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