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Comparison of S. stercoralis Serology Performed on Dried Blood Spots and on Conventional Serum Samples
Background: Dried blood spots (DBS) are used for epidemiological surveys on infectious diseases in settings where limited resources are available. In fact, DBS can help to overcome logistic difficulties for the collection, transport and storage of biological specimens. Objective: To evaluate the acc...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099234/ https://www.ncbi.nlm.nih.gov/pubmed/27877170 http://dx.doi.org/10.3389/fmicb.2016.01778 |
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author | Formenti, Fabio Buonfrate, Dora Prandi, Rosanna Marquez, Monica Caicedo, Cintia Rizzi, Eleonora Guevara, Angel G. Vicuña, Yosselin Huerlo, Francisco R. Perandin, Francesca Bisoffi, Zeno Anselmi, Mariella |
author_facet | Formenti, Fabio Buonfrate, Dora Prandi, Rosanna Marquez, Monica Caicedo, Cintia Rizzi, Eleonora Guevara, Angel G. Vicuña, Yosselin Huerlo, Francisco R. Perandin, Francesca Bisoffi, Zeno Anselmi, Mariella |
author_sort | Formenti, Fabio |
collection | PubMed |
description | Background: Dried blood spots (DBS) are used for epidemiological surveys on infectious diseases in settings where limited resources are available. In fact, DBS can help to overcome logistic difficulties for the collection, transport and storage of biological specimens. Objective: To evaluate the accuracy of Strongyloides stercoralis serology performed on DBS. Methods: A survey was proposed to children attending a school in the village of Borbon, Ecuador, and to their parents/guardians. Each participant gave consent to the collection of both serum and DBS specimens. DBS absorbed on filter papers were analyzed with a commercially available ELISA test for S. stercoralis antibodies, as well as with standard serology. The agreement between the two methods was assessed through the Cohen’s kappa coefficient. Results: The study sample was composed of 174 children and 61 adults, for a total of 235 serum and 235 DBS samples. The serology was positive in 31/235 (13%) serum samples, and in 27/235 (11%) DBS: 4 samples resulted discordant (positive at standard serology). Cohen’s kappa coefficient was 0.921 (95% CI 0.845 – 0.998), indicating a high rate of concordance. Conclusion: DBS are suitable for in field-surveys requiring serological testing for S. stercoralis. |
format | Online Article Text |
id | pubmed-5099234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50992342016-11-22 Comparison of S. stercoralis Serology Performed on Dried Blood Spots and on Conventional Serum Samples Formenti, Fabio Buonfrate, Dora Prandi, Rosanna Marquez, Monica Caicedo, Cintia Rizzi, Eleonora Guevara, Angel G. Vicuña, Yosselin Huerlo, Francisco R. Perandin, Francesca Bisoffi, Zeno Anselmi, Mariella Front Microbiol Microbiology Background: Dried blood spots (DBS) are used for epidemiological surveys on infectious diseases in settings where limited resources are available. In fact, DBS can help to overcome logistic difficulties for the collection, transport and storage of biological specimens. Objective: To evaluate the accuracy of Strongyloides stercoralis serology performed on DBS. Methods: A survey was proposed to children attending a school in the village of Borbon, Ecuador, and to their parents/guardians. Each participant gave consent to the collection of both serum and DBS specimens. DBS absorbed on filter papers were analyzed with a commercially available ELISA test for S. stercoralis antibodies, as well as with standard serology. The agreement between the two methods was assessed through the Cohen’s kappa coefficient. Results: The study sample was composed of 174 children and 61 adults, for a total of 235 serum and 235 DBS samples. The serology was positive in 31/235 (13%) serum samples, and in 27/235 (11%) DBS: 4 samples resulted discordant (positive at standard serology). Cohen’s kappa coefficient was 0.921 (95% CI 0.845 – 0.998), indicating a high rate of concordance. Conclusion: DBS are suitable for in field-surveys requiring serological testing for S. stercoralis. Frontiers Media S.A. 2016-11-08 /pmc/articles/PMC5099234/ /pubmed/27877170 http://dx.doi.org/10.3389/fmicb.2016.01778 Text en Copyright © 2016 Formenti, Buonfrate, Prandi, Marquez, Caicedo, Rizzi, Guevara, Vicuña, Huerlo, Perandin, Bisoffi and Anselmi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Formenti, Fabio Buonfrate, Dora Prandi, Rosanna Marquez, Monica Caicedo, Cintia Rizzi, Eleonora Guevara, Angel G. Vicuña, Yosselin Huerlo, Francisco R. Perandin, Francesca Bisoffi, Zeno Anselmi, Mariella Comparison of S. stercoralis Serology Performed on Dried Blood Spots and on Conventional Serum Samples |
title | Comparison of S. stercoralis Serology Performed on Dried Blood Spots and on Conventional Serum Samples |
title_full | Comparison of S. stercoralis Serology Performed on Dried Blood Spots and on Conventional Serum Samples |
title_fullStr | Comparison of S. stercoralis Serology Performed on Dried Blood Spots and on Conventional Serum Samples |
title_full_unstemmed | Comparison of S. stercoralis Serology Performed on Dried Blood Spots and on Conventional Serum Samples |
title_short | Comparison of S. stercoralis Serology Performed on Dried Blood Spots and on Conventional Serum Samples |
title_sort | comparison of s. stercoralis serology performed on dried blood spots and on conventional serum samples |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099234/ https://www.ncbi.nlm.nih.gov/pubmed/27877170 http://dx.doi.org/10.3389/fmicb.2016.01778 |
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