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Nitidine chloride prevents OVX-induced bone loss via suppressing NFATc1-mediated osteoclast differentiation

Nitidine chloride (NC), a bioactive alkaloid isolated from Zanthoxylum nitidum, has been used as a herbal ingredient in toothpaste that prevents cavities for decades. It also displays potential antitumor and anti-inflammation properties. However, its anticatabolic effect on bone is not known. We inv...

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Autores principales: Liu, Qian, Wang, Tao, Zhou, Lin, Song, Fangming, Qin, An, Feng, Hao Tian, Lin, Xi Xi, Lin, Zhen, Yuan, Jin Bo, Tickner, Jennifer, Liu, Hua Gang, Zheng, Ming Hao, Xu, Jiake, Zhao, Jin Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099608/
https://www.ncbi.nlm.nih.gov/pubmed/27821837
http://dx.doi.org/10.1038/srep36662
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author Liu, Qian
Wang, Tao
Zhou, Lin
Song, Fangming
Qin, An
Feng, Hao Tian
Lin, Xi Xi
Lin, Zhen
Yuan, Jin Bo
Tickner, Jennifer
Liu, Hua Gang
Zheng, Ming Hao
Xu, Jiake
Zhao, Jin Min
author_facet Liu, Qian
Wang, Tao
Zhou, Lin
Song, Fangming
Qin, An
Feng, Hao Tian
Lin, Xi Xi
Lin, Zhen
Yuan, Jin Bo
Tickner, Jennifer
Liu, Hua Gang
Zheng, Ming Hao
Xu, Jiake
Zhao, Jin Min
author_sort Liu, Qian
collection PubMed
description Nitidine chloride (NC), a bioactive alkaloid isolated from Zanthoxylum nitidum, has been used as a herbal ingredient in toothpaste that prevents cavities for decades. It also displays potential antitumor and anti-inflammation properties. However, its anticatabolic effect on bone is not known. We investigated the effect of NC on osteoclastogenesis, bone resorption and RANKL-induced NF-κB and NFATc1 signalling. In mouse-derived bone marrow monocytes (BMMs), NC suppressed RANKL-induced multinucleated tartrate-resistant acid phosphatase (TRAP)-positive osteoclast formation and bone resorption in a dose dependent manner. NC attenuated the expression of osteoclast marker genes including cathepsin K, D2, calcitonin receptor, NFATc1, and TRAP. Further, NC inhibited RANKL-activated NF-κB and NFATc1 signalling pathways. In vivo study revealed that NC abrogated oestrogen deficiency-induced bone loss in ovariectomized mice. Histological analysis showed that the number of osteoclasts was significantly lower in NC-treated groups. Collectively, our data demonstrate that NC suppressed osteoclastogenesis and prevented OVX-induced bone loss by inhibiting RANKL-induced NF-κB and NFATc1 signalling pathways. NC may be a natural and novel treatment for osteoclast-related bone lytic diseases.
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spelling pubmed-50996082016-11-10 Nitidine chloride prevents OVX-induced bone loss via suppressing NFATc1-mediated osteoclast differentiation Liu, Qian Wang, Tao Zhou, Lin Song, Fangming Qin, An Feng, Hao Tian Lin, Xi Xi Lin, Zhen Yuan, Jin Bo Tickner, Jennifer Liu, Hua Gang Zheng, Ming Hao Xu, Jiake Zhao, Jin Min Sci Rep Article Nitidine chloride (NC), a bioactive alkaloid isolated from Zanthoxylum nitidum, has been used as a herbal ingredient in toothpaste that prevents cavities for decades. It also displays potential antitumor and anti-inflammation properties. However, its anticatabolic effect on bone is not known. We investigated the effect of NC on osteoclastogenesis, bone resorption and RANKL-induced NF-κB and NFATc1 signalling. In mouse-derived bone marrow monocytes (BMMs), NC suppressed RANKL-induced multinucleated tartrate-resistant acid phosphatase (TRAP)-positive osteoclast formation and bone resorption in a dose dependent manner. NC attenuated the expression of osteoclast marker genes including cathepsin K, D2, calcitonin receptor, NFATc1, and TRAP. Further, NC inhibited RANKL-activated NF-κB and NFATc1 signalling pathways. In vivo study revealed that NC abrogated oestrogen deficiency-induced bone loss in ovariectomized mice. Histological analysis showed that the number of osteoclasts was significantly lower in NC-treated groups. Collectively, our data demonstrate that NC suppressed osteoclastogenesis and prevented OVX-induced bone loss by inhibiting RANKL-induced NF-κB and NFATc1 signalling pathways. NC may be a natural and novel treatment for osteoclast-related bone lytic diseases. Nature Publishing Group 2016-11-08 /pmc/articles/PMC5099608/ /pubmed/27821837 http://dx.doi.org/10.1038/srep36662 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Qian
Wang, Tao
Zhou, Lin
Song, Fangming
Qin, An
Feng, Hao Tian
Lin, Xi Xi
Lin, Zhen
Yuan, Jin Bo
Tickner, Jennifer
Liu, Hua Gang
Zheng, Ming Hao
Xu, Jiake
Zhao, Jin Min
Nitidine chloride prevents OVX-induced bone loss via suppressing NFATc1-mediated osteoclast differentiation
title Nitidine chloride prevents OVX-induced bone loss via suppressing NFATc1-mediated osteoclast differentiation
title_full Nitidine chloride prevents OVX-induced bone loss via suppressing NFATc1-mediated osteoclast differentiation
title_fullStr Nitidine chloride prevents OVX-induced bone loss via suppressing NFATc1-mediated osteoclast differentiation
title_full_unstemmed Nitidine chloride prevents OVX-induced bone loss via suppressing NFATc1-mediated osteoclast differentiation
title_short Nitidine chloride prevents OVX-induced bone loss via suppressing NFATc1-mediated osteoclast differentiation
title_sort nitidine chloride prevents ovx-induced bone loss via suppressing nfatc1-mediated osteoclast differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099608/
https://www.ncbi.nlm.nih.gov/pubmed/27821837
http://dx.doi.org/10.1038/srep36662
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