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Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies

The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative period...

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Autores principales: Hayakumo, Sae, Arakawa, Shinichi, Takahashi, Masayoshi, Kondo, Keiko, Mano, Yoshihiro, Izumi, Yuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099676/
https://www.ncbi.nlm.nih.gov/pubmed/27877715
http://dx.doi.org/10.1088/1468-6996/15/5/055003
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author Hayakumo, Sae
Arakawa, Shinichi
Takahashi, Masayoshi
Kondo, Keiko
Mano, Yoshihiro
Izumi, Yuichi
author_facet Hayakumo, Sae
Arakawa, Shinichi
Takahashi, Masayoshi
Kondo, Keiko
Mano, Yoshihiro
Izumi, Yuichi
author_sort Hayakumo, Sae
collection PubMed
description The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were evaluated using in vitro time-kill assays. The cytotoxicity of NBW3 was evaluated using three-dimensional human buccal and gingival tissue models. The numbers of colony forming units (CFUs)/mL of P. gingivalis and A. actinomycetemcomitans exposed to NBW3 dropped to below the lower limit of detection (<10 CFUs mL(−1)) after only 0.5 min of exposure. There were only minor decreases in the viability of oral tissue cells after 24 h of exposure to NBW3. These results suggest that NBW3 possesses potent bactericidal activity against representative periodontopathogenic bacteria and is not cytotoxic to cells of human oral tissues. The use of NBW3 as an adjunct to periodontal therapy would be promising.
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spelling pubmed-50996762016-11-22 Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies Hayakumo, Sae Arakawa, Shinichi Takahashi, Masayoshi Kondo, Keiko Mano, Yoshihiro Izumi, Yuichi Sci Technol Adv Mater Papers The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were evaluated using in vitro time-kill assays. The cytotoxicity of NBW3 was evaluated using three-dimensional human buccal and gingival tissue models. The numbers of colony forming units (CFUs)/mL of P. gingivalis and A. actinomycetemcomitans exposed to NBW3 dropped to below the lower limit of detection (<10 CFUs mL(−1)) after only 0.5 min of exposure. There were only minor decreases in the viability of oral tissue cells after 24 h of exposure to NBW3. These results suggest that NBW3 possesses potent bactericidal activity against representative periodontopathogenic bacteria and is not cytotoxic to cells of human oral tissues. The use of NBW3 as an adjunct to periodontal therapy would be promising. Taylor & Francis 2014-09-08 /pmc/articles/PMC5099676/ /pubmed/27877715 http://dx.doi.org/10.1088/1468-6996/15/5/055003 Text en © 2014 National Institute for Materials Science http://creativecommons.org/licenses/by-nc-sa/2.5/ Content from this work may be used under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 2.5 licence (http://creativecommons.org/licenses/by-nc-sa/2.5) . Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
spellingShingle Papers
Hayakumo, Sae
Arakawa, Shinichi
Takahashi, Masayoshi
Kondo, Keiko
Mano, Yoshihiro
Izumi, Yuichi
Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies
title Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies
title_full Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies
title_fullStr Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies
title_full_unstemmed Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies
title_short Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies
title_sort effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies
topic Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099676/
https://www.ncbi.nlm.nih.gov/pubmed/27877715
http://dx.doi.org/10.1088/1468-6996/15/5/055003
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