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Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies
The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative period...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099676/ https://www.ncbi.nlm.nih.gov/pubmed/27877715 http://dx.doi.org/10.1088/1468-6996/15/5/055003 |
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author | Hayakumo, Sae Arakawa, Shinichi Takahashi, Masayoshi Kondo, Keiko Mano, Yoshihiro Izumi, Yuichi |
author_facet | Hayakumo, Sae Arakawa, Shinichi Takahashi, Masayoshi Kondo, Keiko Mano, Yoshihiro Izumi, Yuichi |
author_sort | Hayakumo, Sae |
collection | PubMed |
description | The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were evaluated using in vitro time-kill assays. The cytotoxicity of NBW3 was evaluated using three-dimensional human buccal and gingival tissue models. The numbers of colony forming units (CFUs)/mL of P. gingivalis and A. actinomycetemcomitans exposed to NBW3 dropped to below the lower limit of detection (<10 CFUs mL(−1)) after only 0.5 min of exposure. There were only minor decreases in the viability of oral tissue cells after 24 h of exposure to NBW3. These results suggest that NBW3 possesses potent bactericidal activity against representative periodontopathogenic bacteria and is not cytotoxic to cells of human oral tissues. The use of NBW3 as an adjunct to periodontal therapy would be promising. |
format | Online Article Text |
id | pubmed-5099676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-50996762016-11-22 Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies Hayakumo, Sae Arakawa, Shinichi Takahashi, Masayoshi Kondo, Keiko Mano, Yoshihiro Izumi, Yuichi Sci Technol Adv Mater Papers The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were evaluated using in vitro time-kill assays. The cytotoxicity of NBW3 was evaluated using three-dimensional human buccal and gingival tissue models. The numbers of colony forming units (CFUs)/mL of P. gingivalis and A. actinomycetemcomitans exposed to NBW3 dropped to below the lower limit of detection (<10 CFUs mL(−1)) after only 0.5 min of exposure. There were only minor decreases in the viability of oral tissue cells after 24 h of exposure to NBW3. These results suggest that NBW3 possesses potent bactericidal activity against representative periodontopathogenic bacteria and is not cytotoxic to cells of human oral tissues. The use of NBW3 as an adjunct to periodontal therapy would be promising. Taylor & Francis 2014-09-08 /pmc/articles/PMC5099676/ /pubmed/27877715 http://dx.doi.org/10.1088/1468-6996/15/5/055003 Text en © 2014 National Institute for Materials Science http://creativecommons.org/licenses/by-nc-sa/2.5/ Content from this work may be used under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 2.5 licence (http://creativecommons.org/licenses/by-nc-sa/2.5) . Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. |
spellingShingle | Papers Hayakumo, Sae Arakawa, Shinichi Takahashi, Masayoshi Kondo, Keiko Mano, Yoshihiro Izumi, Yuichi Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies |
title | Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies |
title_full | Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies |
title_fullStr | Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies |
title_full_unstemmed | Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies |
title_short | Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies |
title_sort | effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies |
topic | Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099676/ https://www.ncbi.nlm.nih.gov/pubmed/27877715 http://dx.doi.org/10.1088/1468-6996/15/5/055003 |
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