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Random mutagenesis of the hyperthermophilic archaeon Pyrococcus furiosus using in vitro mariner transposition and natural transformation
Transposition mutagenesis is a powerful tool to identify the function of genes, reveal essential genes and generally to unravel the genetic basis of living organisms. However, transposon-mediated mutagenesis has only been successfully applied to a limited number of archaeal species and has never bee...
Autores principales: | Guschinskaya, Natalia, Brunel, Romain, Tourte, Maxime, Lipscomb, Gina L., Adams, Michael W. W., Oger, Philippe, Charpentier, Xavier |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099854/ https://www.ncbi.nlm.nih.gov/pubmed/27824140 http://dx.doi.org/10.1038/srep36711 |
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