Cargando…

Investigation of Rho-Kinase Expressions and Polymorphisms in Mantle Cell Lymphoma Patients

OBJECTIVE: Mantle cell lymphoma (MCL) is a rare but aggressive form of B-cell non-Hodgkin lymphoma characterized by excessive expression of cyclin D1. Intracellular signaling enzyme Rho-kinase (ROCK) can contribute to cellular migration, proliferation, and differentiation, as well as tumor developme...

Descripción completa

Detalles Bibliográficos
Autores principales: Yanardağ Açık, Didar, Yılmaz, Mehmet, Sarı, İbrahim, Öztuzcu, Serdar, Sayıner, Zeynel A., Subari, Salih, Demiryürek, Abdullah T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Galenos Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100726/
https://www.ncbi.nlm.nih.gov/pubmed/26377148
http://dx.doi.org/10.4274/tjh.2015.0193
_version_ 1782466193774346240
author Yanardağ Açık, Didar
Yılmaz, Mehmet
Sarı, İbrahim
Öztuzcu, Serdar
Sayıner, Zeynel A.
Subari, Salih
Demiryürek, Abdullah T.
author_facet Yanardağ Açık, Didar
Yılmaz, Mehmet
Sarı, İbrahim
Öztuzcu, Serdar
Sayıner, Zeynel A.
Subari, Salih
Demiryürek, Abdullah T.
author_sort Yanardağ Açık, Didar
collection PubMed
description OBJECTIVE: Mantle cell lymphoma (MCL) is a rare but aggressive form of B-cell non-Hodgkin lymphoma characterized by excessive expression of cyclin D1. Intracellular signaling enzyme Rho-kinase (ROCK) can contribute to cellular migration, proliferation, and differentiation, as well as tumor development and metastasis. However, ROCK gene and protein expressions or polymorphisms have never been investigated in MCL patients. The purpose of this study was to investigate the role of ROCK gene and protein expressions in MCL patients. We also examined ROCK2 gene polymorphisms in this study. MATERIALS AND METHODS: A total of 60 patients with MCL and 60 healthy controls were included in this retrospective study. Hematoxylin and eosin-stained lymph node tissue slides in the entire archive were reevaluated and used for immunohistochemistry, gene expression, and polymerase chain reaction studies. RESULTS: In immunohistochemical studies, there were significant increases in ROCK1 (p=0.0009) and ROCK2 (p<0.0001) protein expressions in MCL patients when compared with the control group. Although a marked increase in ROCK1 gene expression (p=0.0215) was noted, no significant change was observed in ROCK2 gene expression in MCL patients. Seven ROCK2 polymorphisms were studied, but the results showed no significant differences between the groups. CONCLUSION: This is the first study to show that ROCK1 gene and ROCK protein expressions may contribute to the development of MCL.
format Online
Article
Text
id pubmed-5100726
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Galenos Publishing
record_format MEDLINE/PubMed
spelling pubmed-51007262016-11-15 Investigation of Rho-Kinase Expressions and Polymorphisms in Mantle Cell Lymphoma Patients Yanardağ Açık, Didar Yılmaz, Mehmet Sarı, İbrahim Öztuzcu, Serdar Sayıner, Zeynel A. Subari, Salih Demiryürek, Abdullah T. Turk J Haematol Research Article OBJECTIVE: Mantle cell lymphoma (MCL) is a rare but aggressive form of B-cell non-Hodgkin lymphoma characterized by excessive expression of cyclin D1. Intracellular signaling enzyme Rho-kinase (ROCK) can contribute to cellular migration, proliferation, and differentiation, as well as tumor development and metastasis. However, ROCK gene and protein expressions or polymorphisms have never been investigated in MCL patients. The purpose of this study was to investigate the role of ROCK gene and protein expressions in MCL patients. We also examined ROCK2 gene polymorphisms in this study. MATERIALS AND METHODS: A total of 60 patients with MCL and 60 healthy controls were included in this retrospective study. Hematoxylin and eosin-stained lymph node tissue slides in the entire archive were reevaluated and used for immunohistochemistry, gene expression, and polymerase chain reaction studies. RESULTS: In immunohistochemical studies, there were significant increases in ROCK1 (p=0.0009) and ROCK2 (p<0.0001) protein expressions in MCL patients when compared with the control group. Although a marked increase in ROCK1 gene expression (p=0.0215) was noted, no significant change was observed in ROCK2 gene expression in MCL patients. Seven ROCK2 polymorphisms were studied, but the results showed no significant differences between the groups. CONCLUSION: This is the first study to show that ROCK1 gene and ROCK protein expressions may contribute to the development of MCL. Galenos Publishing 2016-06 2016-05-16 /pmc/articles/PMC5100726/ /pubmed/26377148 http://dx.doi.org/10.4274/tjh.2015.0193 Text en © Turkish Journal of Hematology, Published by Galenos Publishing. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yanardağ Açık, Didar
Yılmaz, Mehmet
Sarı, İbrahim
Öztuzcu, Serdar
Sayıner, Zeynel A.
Subari, Salih
Demiryürek, Abdullah T.
Investigation of Rho-Kinase Expressions and Polymorphisms in Mantle Cell Lymphoma Patients
title Investigation of Rho-Kinase Expressions and Polymorphisms in Mantle Cell Lymphoma Patients
title_full Investigation of Rho-Kinase Expressions and Polymorphisms in Mantle Cell Lymphoma Patients
title_fullStr Investigation of Rho-Kinase Expressions and Polymorphisms in Mantle Cell Lymphoma Patients
title_full_unstemmed Investigation of Rho-Kinase Expressions and Polymorphisms in Mantle Cell Lymphoma Patients
title_short Investigation of Rho-Kinase Expressions and Polymorphisms in Mantle Cell Lymphoma Patients
title_sort investigation of rho-kinase expressions and polymorphisms in mantle cell lymphoma patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100726/
https://www.ncbi.nlm.nih.gov/pubmed/26377148
http://dx.doi.org/10.4274/tjh.2015.0193
work_keys_str_mv AT yanardagacıkdidar investigationofrhokinaseexpressionsandpolymorphismsinmantlecelllymphomapatients
AT yılmazmehmet investigationofrhokinaseexpressionsandpolymorphismsinmantlecelllymphomapatients
AT sarıibrahim investigationofrhokinaseexpressionsandpolymorphismsinmantlecelllymphomapatients
AT oztuzcuserdar investigationofrhokinaseexpressionsandpolymorphismsinmantlecelllymphomapatients
AT sayınerzeynela investigationofrhokinaseexpressionsandpolymorphismsinmantlecelllymphomapatients
AT subarisalih investigationofrhokinaseexpressionsandpolymorphismsinmantlecelllymphomapatients
AT demiryurekabdullaht investigationofrhokinaseexpressionsandpolymorphismsinmantlecelllymphomapatients