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Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113

Creation of wheat-alien disomic addition lines and localization of desirable genes on alien chromosomes are important for utilization of these genes in genetic improvement of common wheat. In this study, wheat-Agropyron cristatum derivative line 5113 was characterized by genomic in situ hybridizatio...

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Autores principales: Li, Qingfeng, Lu, Yuqing, Pan, Cuili, Yao, Miaomiao, Zhang, Jinpeng, Yang, Xinming, Liu, Weihua, Li, Xiuquan, Xi, Yajun, Li, Lihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100930/
https://www.ncbi.nlm.nih.gov/pubmed/27824906
http://dx.doi.org/10.1371/journal.pone.0165957
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author Li, Qingfeng
Lu, Yuqing
Pan, Cuili
Yao, Miaomiao
Zhang, Jinpeng
Yang, Xinming
Liu, Weihua
Li, Xiuquan
Xi, Yajun
Li, Lihui
author_facet Li, Qingfeng
Lu, Yuqing
Pan, Cuili
Yao, Miaomiao
Zhang, Jinpeng
Yang, Xinming
Liu, Weihua
Li, Xiuquan
Xi, Yajun
Li, Lihui
author_sort Li, Qingfeng
collection PubMed
description Creation of wheat-alien disomic addition lines and localization of desirable genes on alien chromosomes are important for utilization of these genes in genetic improvement of common wheat. In this study, wheat-Agropyron cristatum derivative line 5113 was characterized by genomic in situ hybridization (GISH) and specific-locus amplified fragment sequencing (SLAF-seq), and was demonstrated to be a novel wheat-A. cristatum disomic 6P addition line. Compared with its parent Fukuhokomugi (Fukuho), 5113 displayed multiple elite agronomic traits, including higher uppermost internode/plant height ratio, larger flag leaf, longer spike length, elevated grain number per spike and spikelet number per spike, more kernel number in the middle spikelet, more fertile tiller number per plant, and enhanced resistance to powdery mildew and leaf rust. Genes conferring these elite traits were localized on the A. cristatum 6P chromosome by using SLAF-seq markers and biparental populations (F(1), BC(1)F(1) and BC(1)F(2) populations) produced from the crosses between Fukuho and 5113. Taken together, chromosomal localization of these desirable genes will facilitate transferring of high-yield and high-resistance genes from A. cristatum into common wheat, and serve as the foundation for the utilization of 5113 in wheat breeding.
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spelling pubmed-51009302016-11-18 Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113 Li, Qingfeng Lu, Yuqing Pan, Cuili Yao, Miaomiao Zhang, Jinpeng Yang, Xinming Liu, Weihua Li, Xiuquan Xi, Yajun Li, Lihui PLoS One Research Article Creation of wheat-alien disomic addition lines and localization of desirable genes on alien chromosomes are important for utilization of these genes in genetic improvement of common wheat. In this study, wheat-Agropyron cristatum derivative line 5113 was characterized by genomic in situ hybridization (GISH) and specific-locus amplified fragment sequencing (SLAF-seq), and was demonstrated to be a novel wheat-A. cristatum disomic 6P addition line. Compared with its parent Fukuhokomugi (Fukuho), 5113 displayed multiple elite agronomic traits, including higher uppermost internode/plant height ratio, larger flag leaf, longer spike length, elevated grain number per spike and spikelet number per spike, more kernel number in the middle spikelet, more fertile tiller number per plant, and enhanced resistance to powdery mildew and leaf rust. Genes conferring these elite traits were localized on the A. cristatum 6P chromosome by using SLAF-seq markers and biparental populations (F(1), BC(1)F(1) and BC(1)F(2) populations) produced from the crosses between Fukuho and 5113. Taken together, chromosomal localization of these desirable genes will facilitate transferring of high-yield and high-resistance genes from A. cristatum into common wheat, and serve as the foundation for the utilization of 5113 in wheat breeding. Public Library of Science 2016-11-08 /pmc/articles/PMC5100930/ /pubmed/27824906 http://dx.doi.org/10.1371/journal.pone.0165957 Text en © 2016 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Qingfeng
Lu, Yuqing
Pan, Cuili
Yao, Miaomiao
Zhang, Jinpeng
Yang, Xinming
Liu, Weihua
Li, Xiuquan
Xi, Yajun
Li, Lihui
Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113
title Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113
title_full Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113
title_fullStr Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113
title_full_unstemmed Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113
title_short Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113
title_sort chromosomal localization of genes conferring desirable agronomic traits from wheat-agropyron cristatum disomic addition line 5113
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100930/
https://www.ncbi.nlm.nih.gov/pubmed/27824906
http://dx.doi.org/10.1371/journal.pone.0165957
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