Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India

The aim of this study was to determine sequence types of 34 S. haemolyticus strains isolated from a variety of infections between 2013 and 2016 in India by MLST. The MEGA5.2 software was used to align and compare the nucleotide sequences. The advanced cluster analysis was performed to define the clo...

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Autores principales: Panda, Sasmita, Jena, Smrutiti, Sharma, Savitri, Dhawan, Benu, Nath, Gopal, Singh, Durg Vijai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100990/
https://www.ncbi.nlm.nih.gov/pubmed/27824930
http://dx.doi.org/10.1371/journal.pone.0166193
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author Panda, Sasmita
Jena, Smrutiti
Sharma, Savitri
Dhawan, Benu
Nath, Gopal
Singh, Durg Vijai
author_facet Panda, Sasmita
Jena, Smrutiti
Sharma, Savitri
Dhawan, Benu
Nath, Gopal
Singh, Durg Vijai
author_sort Panda, Sasmita
collection PubMed
description The aim of this study was to determine sequence types of 34 S. haemolyticus strains isolated from a variety of infections between 2013 and 2016 in India by MLST. The MEGA5.2 software was used to align and compare the nucleotide sequences. The advanced cluster analysis was performed to define the clonal complexes. MLST analysis showed 24 new sequence types (ST) among S. haemolyticus isolates, irrespective of sources and place of isolation. The finding of this study allowed to set up an MLST database on the PubMLST.org website using BIGSdb software and made available at http://pubmlst.org/shaemolyticus/. The data of this study thus suggest that MLST can be used to study population structure and diversity among S. haemolyticus isolates.
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spelling pubmed-51009902016-11-18 Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India Panda, Sasmita Jena, Smrutiti Sharma, Savitri Dhawan, Benu Nath, Gopal Singh, Durg Vijai PLoS One Research Article The aim of this study was to determine sequence types of 34 S. haemolyticus strains isolated from a variety of infections between 2013 and 2016 in India by MLST. The MEGA5.2 software was used to align and compare the nucleotide sequences. The advanced cluster analysis was performed to define the clonal complexes. MLST analysis showed 24 new sequence types (ST) among S. haemolyticus isolates, irrespective of sources and place of isolation. The finding of this study allowed to set up an MLST database on the PubMLST.org website using BIGSdb software and made available at http://pubmlst.org/shaemolyticus/. The data of this study thus suggest that MLST can be used to study population structure and diversity among S. haemolyticus isolates. Public Library of Science 2016-11-08 /pmc/articles/PMC5100990/ /pubmed/27824930 http://dx.doi.org/10.1371/journal.pone.0166193 Text en © 2016 Panda et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Panda, Sasmita
Jena, Smrutiti
Sharma, Savitri
Dhawan, Benu
Nath, Gopal
Singh, Durg Vijai
Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India
title Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India
title_full Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India
title_fullStr Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India
title_full_unstemmed Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India
title_short Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India
title_sort identification of novel sequence types among staphylococcus haemolyticus isolated from variety of infections in india
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100990/
https://www.ncbi.nlm.nih.gov/pubmed/27824930
http://dx.doi.org/10.1371/journal.pone.0166193
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