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A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes

BACKGROUND: Peroxisome proliferator-activated receptor γ (PPARγ) is a critical gene that regulates the function of adipocytes. Therefore, studies on the molecular regulation mechanism of PPARγ are important to understand the function of adipose tissue. Twist 1 is another important functional gene in...

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Autores principales: Ren, Rui, Chen, Zhufeng, Zhao, Xia, Sun, Tao, Zhang, Yuchao, Chen, Jie, Lu, Sumei, Ma, Wanshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101646/
https://www.ncbi.nlm.nih.gov/pubmed/27825360
http://dx.doi.org/10.1186/s12944-016-0361-x
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author Ren, Rui
Chen, Zhufeng
Zhao, Xia
Sun, Tao
Zhang, Yuchao
Chen, Jie
Lu, Sumei
Ma, Wanshan
author_facet Ren, Rui
Chen, Zhufeng
Zhao, Xia
Sun, Tao
Zhang, Yuchao
Chen, Jie
Lu, Sumei
Ma, Wanshan
author_sort Ren, Rui
collection PubMed
description BACKGROUND: Peroxisome proliferator-activated receptor γ (PPARγ) is a critical gene that regulates the function of adipocytes. Therefore, studies on the molecular regulation mechanism of PPARγ are important to understand the function of adipose tissue. Twist 1 is another important functional gene in adipose tissue, and hundreds of genes are regulated by Twist 1. The aim of this study was to investigate the regulation of Twist 1 and PPARγ expression in 3T3-L1 mature adipocytes. METHODS: We induced differentiation in 3T3-L1 preadipocytes and examined alterations in Twist 1 and PPARγ expression. We used the PPARγ agonist pioglitazone and the PPARγ antagonist T0070907 to investigate the effect of PPARγ on Twist 1 expression. In addition, we utilized retroviral interference and overexpression of Twist 1 to determine the effects of Twist 1 on PPARγ expression. RESULTS: The expression levels of Twist 1 and PPARγ were induced during differentiation in 3T3-L1 adipocytes. Application of either a PPARγ agonist (pioglitazone) or antagonist (T0070907) influenced Twist 1 expression, with up-regulation of Twist 1 under pioglitazone (1 μM, 24 h) and down-regulation of Twist 1 under T0070907 (100 μM, 24 h) exposure. Furthermore, the retroviral interference of Twist 1 decreased the protein and mRNA expression of PPARγ, while Twist 1 overexpression had the opposite effect. CONCLUSIONS: There was a possible regulatory link between Twist 1 and PPARγ in 3T3-L1 mature adipocytes. This regulatory link enhanced the regulation of PPARγ and may be a functional mechanism of Twist 1 regulation of adipocyte physiology and pathology.
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spelling pubmed-51016462016-11-10 A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes Ren, Rui Chen, Zhufeng Zhao, Xia Sun, Tao Zhang, Yuchao Chen, Jie Lu, Sumei Ma, Wanshan Lipids Health Dis Research BACKGROUND: Peroxisome proliferator-activated receptor γ (PPARγ) is a critical gene that regulates the function of adipocytes. Therefore, studies on the molecular regulation mechanism of PPARγ are important to understand the function of adipose tissue. Twist 1 is another important functional gene in adipose tissue, and hundreds of genes are regulated by Twist 1. The aim of this study was to investigate the regulation of Twist 1 and PPARγ expression in 3T3-L1 mature adipocytes. METHODS: We induced differentiation in 3T3-L1 preadipocytes and examined alterations in Twist 1 and PPARγ expression. We used the PPARγ agonist pioglitazone and the PPARγ antagonist T0070907 to investigate the effect of PPARγ on Twist 1 expression. In addition, we utilized retroviral interference and overexpression of Twist 1 to determine the effects of Twist 1 on PPARγ expression. RESULTS: The expression levels of Twist 1 and PPARγ were induced during differentiation in 3T3-L1 adipocytes. Application of either a PPARγ agonist (pioglitazone) or antagonist (T0070907) influenced Twist 1 expression, with up-regulation of Twist 1 under pioglitazone (1 μM, 24 h) and down-regulation of Twist 1 under T0070907 (100 μM, 24 h) exposure. Furthermore, the retroviral interference of Twist 1 decreased the protein and mRNA expression of PPARγ, while Twist 1 overexpression had the opposite effect. CONCLUSIONS: There was a possible regulatory link between Twist 1 and PPARγ in 3T3-L1 mature adipocytes. This regulatory link enhanced the regulation of PPARγ and may be a functional mechanism of Twist 1 regulation of adipocyte physiology and pathology. BioMed Central 2016-11-08 /pmc/articles/PMC5101646/ /pubmed/27825360 http://dx.doi.org/10.1186/s12944-016-0361-x Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ren, Rui
Chen, Zhufeng
Zhao, Xia
Sun, Tao
Zhang, Yuchao
Chen, Jie
Lu, Sumei
Ma, Wanshan
A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes
title A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes
title_full A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes
title_fullStr A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes
title_full_unstemmed A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes
title_short A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes
title_sort possible regulatory link between twist 1 and pparγ gene regulation in 3t3-l1 adipocytes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101646/
https://www.ncbi.nlm.nih.gov/pubmed/27825360
http://dx.doi.org/10.1186/s12944-016-0361-x
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