Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells

Numerous clinical therapeutic agents have been identified as DNA damaging. The present study revealed that isoproterenol (Iso) resulted in DNA damage in vascular smooth muscle cells (VSMCs) and increased the levels of intracellular oxygen free radicals. Administration of chlorogenic acid (CGA) inhib...

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Autores principales: Wang, Jingshuai, Li, Jiyang, Liu, Jie, Xu, Mengjiao, Tong, Xiaowen, Wang, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101879/
https://www.ncbi.nlm.nih.gov/pubmed/27634104
http://dx.doi.org/10.3892/mmr.2016.5743
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author Wang, Jingshuai
Li, Jiyang
Liu, Jie
Xu, Mengjiao
Tong, Xiaowen
Wang, Jianjun
author_facet Wang, Jingshuai
Li, Jiyang
Liu, Jie
Xu, Mengjiao
Tong, Xiaowen
Wang, Jianjun
author_sort Wang, Jingshuai
collection PubMed
description Numerous clinical therapeutic agents have been identified as DNA damaging. The present study revealed that isoproterenol (Iso) resulted in DNA damage in vascular smooth muscle cells (VSMCs) and increased the levels of intracellular oxygen free radicals. Administration of chlorogenic acid (CGA) inhibited this effect. Pretreatment with CGA abrogated the increase in protein expression levels of γ-H2A histone family member X, phosphorylated ataxia telangiectasia mutated, phosphorylated Rad3-related protein, breast cancer 1 and C-terminal Src homologous kinase induced by Iso. In addition, the increase in levels of intracellular reactive oxygen species (ROS) induced by Iso was inhibited by CGA pretreatment in a dose-dependent manner. The results of the present study suggest that CGA may inhibit Iso-induced VSMC damage via the suppression of ROS generation. Therefore, CGA may be a novel agent for the treatment of vascular diseases.
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spelling pubmed-51018792016-11-22 Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells Wang, Jingshuai Li, Jiyang Liu, Jie Xu, Mengjiao Tong, Xiaowen Wang, Jianjun Mol Med Rep Articles Numerous clinical therapeutic agents have been identified as DNA damaging. The present study revealed that isoproterenol (Iso) resulted in DNA damage in vascular smooth muscle cells (VSMCs) and increased the levels of intracellular oxygen free radicals. Administration of chlorogenic acid (CGA) inhibited this effect. Pretreatment with CGA abrogated the increase in protein expression levels of γ-H2A histone family member X, phosphorylated ataxia telangiectasia mutated, phosphorylated Rad3-related protein, breast cancer 1 and C-terminal Src homologous kinase induced by Iso. In addition, the increase in levels of intracellular reactive oxygen species (ROS) induced by Iso was inhibited by CGA pretreatment in a dose-dependent manner. The results of the present study suggest that CGA may inhibit Iso-induced VSMC damage via the suppression of ROS generation. Therefore, CGA may be a novel agent for the treatment of vascular diseases. D.A. Spandidos 2016-11 2016-09-15 /pmc/articles/PMC5101879/ /pubmed/27634104 http://dx.doi.org/10.3892/mmr.2016.5743 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Jingshuai
Li, Jiyang
Liu, Jie
Xu, Mengjiao
Tong, Xiaowen
Wang, Jianjun
Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells
title Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells
title_full Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells
title_fullStr Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells
title_full_unstemmed Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells
title_short Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells
title_sort chlorogenic acid prevents isoproterenol-induced dna damage in vascular smooth muscle cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101879/
https://www.ncbi.nlm.nih.gov/pubmed/27634104
http://dx.doi.org/10.3892/mmr.2016.5743
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AT tongxiaowen chlorogenicacidpreventsisoproterenolinduceddnadamageinvascularsmoothmusclecells
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