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Activation of CYCD7;1 in the central cell and early endosperm overcomes cell‐cycle arrest in the Arabidopsis female gametophyte, and promotes early endosperm and embryo development
In angiosperms, double fertilization of the egg and central cell of the megagametophyte leads to the development of the embryo and endosperm, respectively. Control of cell cycle progression in the megagametophyte is essential for successful fertilization and development. Central cell‐targeted expres...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5102630/ https://www.ncbi.nlm.nih.gov/pubmed/26261067 http://dx.doi.org/10.1111/tpj.12957 |
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author | Sornay, Emily Forzani, Céline Forero‐Vargas, Manuel Dewitte, Walter Murray, James A.H. |
author_facet | Sornay, Emily Forzani, Céline Forero‐Vargas, Manuel Dewitte, Walter Murray, James A.H. |
author_sort | Sornay, Emily |
collection | PubMed |
description | In angiosperms, double fertilization of the egg and central cell of the megagametophyte leads to the development of the embryo and endosperm, respectively. Control of cell cycle progression in the megagametophyte is essential for successful fertilization and development. Central cell‐targeted expression of the D‐type cyclin CYCD7;1 ((end)CYCD7;1) using the imprinted FWA promoter overcomes cycle arrest of the central cell in the Arabidopsis female gametophyte in the unfertilized ovule, leading to multinucleate central cells at high frequency. Unlike FERTILIZATION‐INDEPENDENT SEED (fis) mutants, but similar to lethal RETINOBLASTOMA‐RELATED (rbr) mutants, no seed coat development is triggered. Unlike the case with loss of rbr, post‐fertilization (end)CYCD7;1 in the endosperm enhances the number of nuclei during syncytial endosperm development and induces the partial abortion of developing seeds, associated with the enhanced size of the surviving seeds. The frequency of lethality was less than the frequency of multinucleate central cells, indicating that these aspects are not causally linked. These larger seeds contain larger embryos composed of more cells of wild‐type size, surrounded by a seed coat composed of more cells. Seedlings arising from these larger seeds displayed faster seedling establishment and early growth. Similarly, two different embryo‐lethal mutants also conferred enlarged seed size in surviving siblings, consistent with seed size increase being a general response to sibling lethality, although the cellular mechanisms were found to be distinct. Our data suggest that tight control of CYCD activity in the central cell and in the developing endosperm is required for optimal seed formation. |
format | Online Article Text |
id | pubmed-5102630 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51026302016-11-16 Activation of CYCD7;1 in the central cell and early endosperm overcomes cell‐cycle arrest in the Arabidopsis female gametophyte, and promotes early endosperm and embryo development Sornay, Emily Forzani, Céline Forero‐Vargas, Manuel Dewitte, Walter Murray, James A.H. Plant J Original Articles In angiosperms, double fertilization of the egg and central cell of the megagametophyte leads to the development of the embryo and endosperm, respectively. Control of cell cycle progression in the megagametophyte is essential for successful fertilization and development. Central cell‐targeted expression of the D‐type cyclin CYCD7;1 ((end)CYCD7;1) using the imprinted FWA promoter overcomes cycle arrest of the central cell in the Arabidopsis female gametophyte in the unfertilized ovule, leading to multinucleate central cells at high frequency. Unlike FERTILIZATION‐INDEPENDENT SEED (fis) mutants, but similar to lethal RETINOBLASTOMA‐RELATED (rbr) mutants, no seed coat development is triggered. Unlike the case with loss of rbr, post‐fertilization (end)CYCD7;1 in the endosperm enhances the number of nuclei during syncytial endosperm development and induces the partial abortion of developing seeds, associated with the enhanced size of the surviving seeds. The frequency of lethality was less than the frequency of multinucleate central cells, indicating that these aspects are not causally linked. These larger seeds contain larger embryos composed of more cells of wild‐type size, surrounded by a seed coat composed of more cells. Seedlings arising from these larger seeds displayed faster seedling establishment and early growth. Similarly, two different embryo‐lethal mutants also conferred enlarged seed size in surviving siblings, consistent with seed size increase being a general response to sibling lethality, although the cellular mechanisms were found to be distinct. Our data suggest that tight control of CYCD activity in the central cell and in the developing endosperm is required for optimal seed formation. John Wiley and Sons Inc. 2015-10-01 2015-10 /pmc/articles/PMC5102630/ /pubmed/26261067 http://dx.doi.org/10.1111/tpj.12957 Text en © 2015 The Authors The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Sornay, Emily Forzani, Céline Forero‐Vargas, Manuel Dewitte, Walter Murray, James A.H. Activation of CYCD7;1 in the central cell and early endosperm overcomes cell‐cycle arrest in the Arabidopsis female gametophyte, and promotes early endosperm and embryo development |
title | Activation of CYCD7;1 in the central cell and early endosperm overcomes cell‐cycle arrest in the Arabidopsis female gametophyte, and promotes early endosperm and embryo development |
title_full | Activation of CYCD7;1 in the central cell and early endosperm overcomes cell‐cycle arrest in the Arabidopsis female gametophyte, and promotes early endosperm and embryo development |
title_fullStr | Activation of CYCD7;1 in the central cell and early endosperm overcomes cell‐cycle arrest in the Arabidopsis female gametophyte, and promotes early endosperm and embryo development |
title_full_unstemmed | Activation of CYCD7;1 in the central cell and early endosperm overcomes cell‐cycle arrest in the Arabidopsis female gametophyte, and promotes early endosperm and embryo development |
title_short | Activation of CYCD7;1 in the central cell and early endosperm overcomes cell‐cycle arrest in the Arabidopsis female gametophyte, and promotes early endosperm and embryo development |
title_sort | activation of cycd7;1 in the central cell and early endosperm overcomes cell‐cycle arrest in the arabidopsis female gametophyte, and promotes early endosperm and embryo development |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5102630/ https://www.ncbi.nlm.nih.gov/pubmed/26261067 http://dx.doi.org/10.1111/tpj.12957 |
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