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Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F
The F plasmid is the foremost representative of a large group of conjugative plasmids, prevalent in Escherichia coli, and widely distributed among the Enterobacteriaceae. These plasmids are of clinical relevance, given their frequent association with virulence determinants, colicins, and antibiotic...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5102898/ https://www.ncbi.nlm.nih.gov/pubmed/27891505 http://dx.doi.org/10.3389/fmolb.2016.00071 |
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author | Fernandez-Lopez, Raul de Toro, Maria Moncalian, Gabriel Garcillan-Barcia, M. Pilar de la Cruz, Fernando |
author_facet | Fernandez-Lopez, Raul de Toro, Maria Moncalian, Gabriel Garcillan-Barcia, M. Pilar de la Cruz, Fernando |
author_sort | Fernandez-Lopez, Raul |
collection | PubMed |
description | The F plasmid is the foremost representative of a large group of conjugative plasmids, prevalent in Escherichia coli, and widely distributed among the Enterobacteriaceae. These plasmids are of clinical relevance, given their frequent association with virulence determinants, colicins, and antibiotic resistance genes. Originally defined by their sensitivity to certain male-specific phages, IncF plasmids share a conserved conjugative system and regulatory circuits. In order to determine whether the genetic architecture and regulation circuits are preserved among these plasmids, we analyzed the natural diversity of F-like plasmids. Using the relaxase as a phylogenetic marker, we identified 256 plasmids belonging to the IncF/ MOB(F12)group, present as complete DNA sequences in the NCBI database. By comparative genomics, we identified five major groups of F-like plasmids. Each shows a particular operon structure and alternate regulatory systems. Results show that the IncF/MOB(F12) conjugation gene cluster conforms a diverse and ancient group, which evolved alternative regulatory schemes in its adaptation to different environments and bacterial hosts. |
format | Online Article Text |
id | pubmed-5102898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51028982016-11-25 Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F Fernandez-Lopez, Raul de Toro, Maria Moncalian, Gabriel Garcillan-Barcia, M. Pilar de la Cruz, Fernando Front Mol Biosci Molecular Biosciences The F plasmid is the foremost representative of a large group of conjugative plasmids, prevalent in Escherichia coli, and widely distributed among the Enterobacteriaceae. These plasmids are of clinical relevance, given their frequent association with virulence determinants, colicins, and antibiotic resistance genes. Originally defined by their sensitivity to certain male-specific phages, IncF plasmids share a conserved conjugative system and regulatory circuits. In order to determine whether the genetic architecture and regulation circuits are preserved among these plasmids, we analyzed the natural diversity of F-like plasmids. Using the relaxase as a phylogenetic marker, we identified 256 plasmids belonging to the IncF/ MOB(F12)group, present as complete DNA sequences in the NCBI database. By comparative genomics, we identified five major groups of F-like plasmids. Each shows a particular operon structure and alternate regulatory systems. Results show that the IncF/MOB(F12) conjugation gene cluster conforms a diverse and ancient group, which evolved alternative regulatory schemes in its adaptation to different environments and bacterial hosts. Frontiers Media S.A. 2016-11-10 /pmc/articles/PMC5102898/ /pubmed/27891505 http://dx.doi.org/10.3389/fmolb.2016.00071 Text en Copyright © 2016 Fernandez-Lopez, de Toro, Moncalian, Garcillan-Barcia and de la Cruz. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Fernandez-Lopez, Raul de Toro, Maria Moncalian, Gabriel Garcillan-Barcia, M. Pilar de la Cruz, Fernando Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F |
title | Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F |
title_full | Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F |
title_fullStr | Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F |
title_full_unstemmed | Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F |
title_short | Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F |
title_sort | comparative genomics of the conjugation region of f-like plasmids: five shades of f |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5102898/ https://www.ncbi.nlm.nih.gov/pubmed/27891505 http://dx.doi.org/10.3389/fmolb.2016.00071 |
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