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Relation of peritubular capillary features to class of lupus nephritis

BACKGROUND: Experimental studies have linked peritubular capillary (PTC) loss with progression of chronic kidney disease. Minimal information on PTC in lupus nephritis (LN) has been reported. We therefore evaluated the PTC area in different classes of LN and determined if specific clinical character...

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Autores principales: Anutrakulchai, Sirirat, Titipungul, Tanin, Pattay, Thanyaluk, Mesung, Putachart, Puapairoj, Anucha, Sirivongs, Dhavee, Pongsakul, Cholatip, Futrakul, Prasit, Thinkhamrop, Bandit, Johnson, Richard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103390/
https://www.ncbi.nlm.nih.gov/pubmed/27829387
http://dx.doi.org/10.1186/s12882-016-0388-2
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author Anutrakulchai, Sirirat
Titipungul, Tanin
Pattay, Thanyaluk
Mesung, Putachart
Puapairoj, Anucha
Sirivongs, Dhavee
Pongsakul, Cholatip
Futrakul, Prasit
Thinkhamrop, Bandit
Johnson, Richard J.
author_facet Anutrakulchai, Sirirat
Titipungul, Tanin
Pattay, Thanyaluk
Mesung, Putachart
Puapairoj, Anucha
Sirivongs, Dhavee
Pongsakul, Cholatip
Futrakul, Prasit
Thinkhamrop, Bandit
Johnson, Richard J.
author_sort Anutrakulchai, Sirirat
collection PubMed
description BACKGROUND: Experimental studies have linked peritubular capillary (PTC) loss with progression of chronic kidney disease. Minimal information on PTC in lupus nephritis (LN) has been reported. We therefore evaluated the PTC area in different classes of LN and determined if specific clinical characteristics correlated with PTC changes. METHODS: Renal biopsies of 253 subjects with LN (categorized using the ISN/RPS 2003 classification) and 13 normal renal donors (the controls) were retrospectively evaluated for PTC morphology by staining for CD31 with immunohistochemistry method. The percent positive area of PTC (% PTC) was correlated with serum and urinary measures of renal function and renal pathology. RESULTS: Significant PTC loss was observed in all classes of LN compared to controls. The % PTC area was highest in controls (7.64±1.48 %) with levels of 1.95±1.50, 4.16±3.85, 4.19±4.45, 5.02±1.79, and 4.45±3.75 in classes II, III, IV, IV combined with V and V, respectively (all p values < 0.05). The lowest PTC density was observed in class II LN, but this may be because some cases with worse classes of LN showed increased PTC density due to abnormally dilated capillaries associated with acute inflammation and angiogenesis. %PTC was increased in those with hematuria (5.8±5.2 vs. 3.6±3.4 %, red blood cells 3-10 vs. < 3 cells/high power field, p < 0.05) and was reduced in those with a moderately declined renal function (3.29±3.40 vs. 4.42±4.12, eGFR 15-59 vs. ≥ 60 ml/min/1.73 m2, p < 0.05). Nephrotic-range proteinuria also trended to be associated with lower PTC density although it did not reach statistical significance (3.1±2.6 vs. 4.9±4.5, p= 0.067). CONCLUSIONS: LN is associated with PTC loss and the severity correlates with reduced renal function. Further studies are needed to investigate whether a loss of PTC can predict long term renal outcomes in LN. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12882-016-0388-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-51033902016-11-10 Relation of peritubular capillary features to class of lupus nephritis Anutrakulchai, Sirirat Titipungul, Tanin Pattay, Thanyaluk Mesung, Putachart Puapairoj, Anucha Sirivongs, Dhavee Pongsakul, Cholatip Futrakul, Prasit Thinkhamrop, Bandit Johnson, Richard J. BMC Nephrol Research Article BACKGROUND: Experimental studies have linked peritubular capillary (PTC) loss with progression of chronic kidney disease. Minimal information on PTC in lupus nephritis (LN) has been reported. We therefore evaluated the PTC area in different classes of LN and determined if specific clinical characteristics correlated with PTC changes. METHODS: Renal biopsies of 253 subjects with LN (categorized using the ISN/RPS 2003 classification) and 13 normal renal donors (the controls) were retrospectively evaluated for PTC morphology by staining for CD31 with immunohistochemistry method. The percent positive area of PTC (% PTC) was correlated with serum and urinary measures of renal function and renal pathology. RESULTS: Significant PTC loss was observed in all classes of LN compared to controls. The % PTC area was highest in controls (7.64±1.48 %) with levels of 1.95±1.50, 4.16±3.85, 4.19±4.45, 5.02±1.79, and 4.45±3.75 in classes II, III, IV, IV combined with V and V, respectively (all p values < 0.05). The lowest PTC density was observed in class II LN, but this may be because some cases with worse classes of LN showed increased PTC density due to abnormally dilated capillaries associated with acute inflammation and angiogenesis. %PTC was increased in those with hematuria (5.8±5.2 vs. 3.6±3.4 %, red blood cells 3-10 vs. < 3 cells/high power field, p < 0.05) and was reduced in those with a moderately declined renal function (3.29±3.40 vs. 4.42±4.12, eGFR 15-59 vs. ≥ 60 ml/min/1.73 m2, p < 0.05). Nephrotic-range proteinuria also trended to be associated with lower PTC density although it did not reach statistical significance (3.1±2.6 vs. 4.9±4.5, p= 0.067). CONCLUSIONS: LN is associated with PTC loss and the severity correlates with reduced renal function. Further studies are needed to investigate whether a loss of PTC can predict long term renal outcomes in LN. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12882-016-0388-2) contains supplementary material, which is available to authorized users. BioMed Central 2016-11-09 /pmc/articles/PMC5103390/ /pubmed/27829387 http://dx.doi.org/10.1186/s12882-016-0388-2 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Anutrakulchai, Sirirat
Titipungul, Tanin
Pattay, Thanyaluk
Mesung, Putachart
Puapairoj, Anucha
Sirivongs, Dhavee
Pongsakul, Cholatip
Futrakul, Prasit
Thinkhamrop, Bandit
Johnson, Richard J.
Relation of peritubular capillary features to class of lupus nephritis
title Relation of peritubular capillary features to class of lupus nephritis
title_full Relation of peritubular capillary features to class of lupus nephritis
title_fullStr Relation of peritubular capillary features to class of lupus nephritis
title_full_unstemmed Relation of peritubular capillary features to class of lupus nephritis
title_short Relation of peritubular capillary features to class of lupus nephritis
title_sort relation of peritubular capillary features to class of lupus nephritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103390/
https://www.ncbi.nlm.nih.gov/pubmed/27829387
http://dx.doi.org/10.1186/s12882-016-0388-2
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