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Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana

BACKGROUND: Rotaviruses with G6P[14] specificity are mostly isolated in cattle and have been established as a rare cause of gastroenteritis in humans. This study reports the first detection of G6P[14] rotavirus strain in Ghana from the stool of an infant during a hospital-based rotavirus surveillanc...

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Autores principales: Damanka, Susan, Lartey, Belinda, Agbemabiese, Chantal, Dennis, Francis E., Adiku, Theophilus, Nyarko, Kofi, Ofori, Michael, Armah, George E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103419/
https://www.ncbi.nlm.nih.gov/pubmed/27832798
http://dx.doi.org/10.1186/s12985-016-0643-y
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author Damanka, Susan
Lartey, Belinda
Agbemabiese, Chantal
Dennis, Francis E.
Adiku, Theophilus
Nyarko, Kofi
Ofori, Michael
Armah, George E.
author_facet Damanka, Susan
Lartey, Belinda
Agbemabiese, Chantal
Dennis, Francis E.
Adiku, Theophilus
Nyarko, Kofi
Ofori, Michael
Armah, George E.
author_sort Damanka, Susan
collection PubMed
description BACKGROUND: Rotaviruses with G6P[14] specificity are mostly isolated in cattle and have been established as a rare cause of gastroenteritis in humans. This study reports the first detection of G6P[14] rotavirus strain in Ghana from the stool of an infant during a hospital-based rotavirus surveillance study in 2010. METHODS: Viral RNA was extracted and rotavirus VP7 and VP4 genes amplified by one step RT-PCR using gene-specific primers. The DNA was purified, sequenced and genotypes determined using BLAST and RotaC v2.0. Phylogenetic tree was constructed using maximum likelihood method in MEGA v6.06 software and statistically supported by bootstrapping with 1000 replicates. Phylogenetic distances were calculated using the Kimura-2 parameter model. RESULTS: The study strain, GHA-M0084/2010 was characterised as G6P[14]. The VP7 gene of the Ghanaian strain clustered in G6 lineage-III together with artiodactyl and human rotavirus (HRV) strains. It exhibited the highest nucleotide (88.1 %) and amino acid (86.9 %) sequence identity with Belgian HRV strain, B10925. The VP8* fragment of the VP4 gene was closely related to HRV strains detected in France, Italy, Spain and Belgium. It exhibited the strongest nucleotide sequence identity (87.9 %) with HRV strains, PA169 and PR/1300 (Italy) and the strongest amino acid sequence identity (89.3 %) with HRV strain R2775/FRA/07 (France). CONCLUSION: The study reports the first detection of G6P[14] HRV strain in an infant in Ghana. The detection of G6P[14], an unusual strain pre-vaccine introduction in Ghana, suggests a potential compromise of vaccine effectiveness and indicates the necessity for continuous surveillance in the post vaccine era. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-016-0643-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-51034192016-11-10 Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana Damanka, Susan Lartey, Belinda Agbemabiese, Chantal Dennis, Francis E. Adiku, Theophilus Nyarko, Kofi Ofori, Michael Armah, George E. Virol J Short Report BACKGROUND: Rotaviruses with G6P[14] specificity are mostly isolated in cattle and have been established as a rare cause of gastroenteritis in humans. This study reports the first detection of G6P[14] rotavirus strain in Ghana from the stool of an infant during a hospital-based rotavirus surveillance study in 2010. METHODS: Viral RNA was extracted and rotavirus VP7 and VP4 genes amplified by one step RT-PCR using gene-specific primers. The DNA was purified, sequenced and genotypes determined using BLAST and RotaC v2.0. Phylogenetic tree was constructed using maximum likelihood method in MEGA v6.06 software and statistically supported by bootstrapping with 1000 replicates. Phylogenetic distances were calculated using the Kimura-2 parameter model. RESULTS: The study strain, GHA-M0084/2010 was characterised as G6P[14]. The VP7 gene of the Ghanaian strain clustered in G6 lineage-III together with artiodactyl and human rotavirus (HRV) strains. It exhibited the highest nucleotide (88.1 %) and amino acid (86.9 %) sequence identity with Belgian HRV strain, B10925. The VP8* fragment of the VP4 gene was closely related to HRV strains detected in France, Italy, Spain and Belgium. It exhibited the strongest nucleotide sequence identity (87.9 %) with HRV strains, PA169 and PR/1300 (Italy) and the strongest amino acid sequence identity (89.3 %) with HRV strain R2775/FRA/07 (France). CONCLUSION: The study reports the first detection of G6P[14] HRV strain in an infant in Ghana. The detection of G6P[14], an unusual strain pre-vaccine introduction in Ghana, suggests a potential compromise of vaccine effectiveness and indicates the necessity for continuous surveillance in the post vaccine era. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-016-0643-y) contains supplementary material, which is available to authorized users. BioMed Central 2016-11-10 /pmc/articles/PMC5103419/ /pubmed/27832798 http://dx.doi.org/10.1186/s12985-016-0643-y Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Damanka, Susan
Lartey, Belinda
Agbemabiese, Chantal
Dennis, Francis E.
Adiku, Theophilus
Nyarko, Kofi
Ofori, Michael
Armah, George E.
Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana
title Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana
title_full Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana
title_fullStr Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana
title_full_unstemmed Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana
title_short Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana
title_sort detection of the first g6p[14] human rotavirus strain in an infant with diarrhoea in ghana
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103419/
https://www.ncbi.nlm.nih.gov/pubmed/27832798
http://dx.doi.org/10.1186/s12985-016-0643-y
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