Cargando…
Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy
The present retrospective study aimed to examine the association between the expression of long non-protein-coding RNAs (lncRNAs) and clinical prognosis in the pretreatment formalin-fixed, paraffin-embedded (FFPE) tissue samples of cervical squamous cell carcinoma patients that underwent platinum-ba...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103900/ https://www.ncbi.nlm.nih.gov/pubmed/27899965 http://dx.doi.org/10.3892/ol.2016.5054 |
_version_ | 1782466660001644544 |
---|---|
author | Kobayashi, Reiko Miyagawa, Ryu Yamashita, Hideomi Morikawa, Teppei Okuma, Kae Fukayama, Masashi Ohtomo, Kuni Nakagawa, Keiichi |
author_facet | Kobayashi, Reiko Miyagawa, Ryu Yamashita, Hideomi Morikawa, Teppei Okuma, Kae Fukayama, Masashi Ohtomo, Kuni Nakagawa, Keiichi |
author_sort | Kobayashi, Reiko |
collection | PubMed |
description | The present retrospective study aimed to examine the association between the expression of long non-protein-coding RNAs (lncRNAs) and clinical prognosis in the pretreatment formalin-fixed, paraffin-embedded (FFPE) tissue samples of cervical squamous cell carcinoma patients that underwent platinum-based chemoradiation therapy. Between 2001 and 2013, 49 consecutive patients with squamous cell cervical carcinoma were selected for the present study (median follow-up period, 44.1 months). The patients possessed an International Federation of Gynecology and Obstetrics stage of IB1/IIA1 (with pelvic lymph node metastasis), IB2 or IIA2-IVA, and had been treated with definitive chemoradiation therapy. The pretreatment FFPE tumor biopsies of the patients obtained diagnosis were used for analysis. Total RNAs were extracted from the FFPE tumor tissues and reverse transcription-quantitative polymerase chain reaction was performed to examine the expression level of lncRNAs. The expression level of X inactive-specific transcript (XIST) demonstrated a significant association with the overall survival rate (P=0.014). The 4-year overall survival rates were 87.1 and 54.4% in the high and low XIST expression groups, respectively. Since the expression of XIST is associated with the overall survival rate, this lncRNA has the potential to become a predictor for the prognosis of cervical squamous cell carcinoma patients that are treated with chemoradiation therapy. Additional studies are required to investigate the underlying mechanisms of XIST that are associated with prognosis. |
format | Online Article Text |
id | pubmed-5103900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-51039002016-11-29 Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy Kobayashi, Reiko Miyagawa, Ryu Yamashita, Hideomi Morikawa, Teppei Okuma, Kae Fukayama, Masashi Ohtomo, Kuni Nakagawa, Keiichi Oncol Lett Articles The present retrospective study aimed to examine the association between the expression of long non-protein-coding RNAs (lncRNAs) and clinical prognosis in the pretreatment formalin-fixed, paraffin-embedded (FFPE) tissue samples of cervical squamous cell carcinoma patients that underwent platinum-based chemoradiation therapy. Between 2001 and 2013, 49 consecutive patients with squamous cell cervical carcinoma were selected for the present study (median follow-up period, 44.1 months). The patients possessed an International Federation of Gynecology and Obstetrics stage of IB1/IIA1 (with pelvic lymph node metastasis), IB2 or IIA2-IVA, and had been treated with definitive chemoradiation therapy. The pretreatment FFPE tumor biopsies of the patients obtained diagnosis were used for analysis. Total RNAs were extracted from the FFPE tumor tissues and reverse transcription-quantitative polymerase chain reaction was performed to examine the expression level of lncRNAs. The expression level of X inactive-specific transcript (XIST) demonstrated a significant association with the overall survival rate (P=0.014). The 4-year overall survival rates were 87.1 and 54.4% in the high and low XIST expression groups, respectively. Since the expression of XIST is associated with the overall survival rate, this lncRNA has the potential to become a predictor for the prognosis of cervical squamous cell carcinoma patients that are treated with chemoradiation therapy. Additional studies are required to investigate the underlying mechanisms of XIST that are associated with prognosis. D.A. Spandidos 2016-11 2016-08-26 /pmc/articles/PMC5103900/ /pubmed/27899965 http://dx.doi.org/10.3892/ol.2016.5054 Text en Copyright: © Kobayashi et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Kobayashi, Reiko Miyagawa, Ryu Yamashita, Hideomi Morikawa, Teppei Okuma, Kae Fukayama, Masashi Ohtomo, Kuni Nakagawa, Keiichi Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy |
title | Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy |
title_full | Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy |
title_fullStr | Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy |
title_full_unstemmed | Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy |
title_short | Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy |
title_sort | increased expression of long non-coding rna xist predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103900/ https://www.ncbi.nlm.nih.gov/pubmed/27899965 http://dx.doi.org/10.3892/ol.2016.5054 |
work_keys_str_mv | AT kobayashireiko increasedexpressionoflongnoncodingrnaxistpredictsfavorableprognosisofcervicalsquamouscellcarcinomasubsequenttodefinitivechemoradiationtherapy AT miyagawaryu increasedexpressionoflongnoncodingrnaxistpredictsfavorableprognosisofcervicalsquamouscellcarcinomasubsequenttodefinitivechemoradiationtherapy AT yamashitahideomi increasedexpressionoflongnoncodingrnaxistpredictsfavorableprognosisofcervicalsquamouscellcarcinomasubsequenttodefinitivechemoradiationtherapy AT morikawateppei increasedexpressionoflongnoncodingrnaxistpredictsfavorableprognosisofcervicalsquamouscellcarcinomasubsequenttodefinitivechemoradiationtherapy AT okumakae increasedexpressionoflongnoncodingrnaxistpredictsfavorableprognosisofcervicalsquamouscellcarcinomasubsequenttodefinitivechemoradiationtherapy AT fukayamamasashi increasedexpressionoflongnoncodingrnaxistpredictsfavorableprognosisofcervicalsquamouscellcarcinomasubsequenttodefinitivechemoradiationtherapy AT ohtomokuni increasedexpressionoflongnoncodingrnaxistpredictsfavorableprognosisofcervicalsquamouscellcarcinomasubsequenttodefinitivechemoradiationtherapy AT nakagawakeiichi increasedexpressionoflongnoncodingrnaxistpredictsfavorableprognosisofcervicalsquamouscellcarcinomasubsequenttodefinitivechemoradiationtherapy |