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Evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells

The aim of the present study was to evaluate the efficacy of paclitaxel combined with curcumin (CUR) against drug resistance in ovarian cancer cells. PLGA-phospholipid-PEG nanoparticles were prepared using the nano precipitation method. The size and morphology of the nanoparticles were determined us...

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Autores principales: Liu, Zeng, Zhu, Yuan-Yuan, Li, Zhao-Yuan, Ning, Si-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104213/
https://www.ncbi.nlm.nih.gov/pubmed/27895754
http://dx.doi.org/10.3892/ol.2016.5192
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author Liu, Zeng
Zhu, Yuan-Yuan
Li, Zhao-Yuan
Ning, Si-Qing
author_facet Liu, Zeng
Zhu, Yuan-Yuan
Li, Zhao-Yuan
Ning, Si-Qing
author_sort Liu, Zeng
collection PubMed
description The aim of the present study was to evaluate the efficacy of paclitaxel combined with curcumin (CUR) against drug resistance in ovarian cancer cells. PLGA-phospholipid-PEG nanoparticles were prepared using the nano precipitation method. The size and morphology of the nanoparticles were determined using a transmission electron microscope and particle size analyzer. The encapsulation efficiency of nanoparticles was determined using the ultrafiltration centrifugation method. The dialysis method was used to study the release of PLGA-phospholipid-PEG nanoparticles. ADM was used to induce the A2780 cell line (human ovarian cancer cell line) to establish the model of the multidrug-resistant (MDR) cell line, and the protein activity of P-glycoprotein (P-gp) in the A2780 cell line and A2780/ADM resistant cell line was determined using western blot analysis. The results showed that, the prepared nanoparticles were uniform in size, with a size of approximately 100 nm, and round in shape. Additionally, the nanoparticles had a more gentle and slow release than the free drug release. The results of the protein trace printing experiment showed that the P-gp content of the drug-resistant cell line was significantly reduced by the CUR nanoparticles. In conclusion, PLGA-phospholipid nanoparticles containing taxol and CUR have improved solubility and stability together with a slow release effect. In addition, CUR was able to overcome the MDR of tumor cells by elevating the paclitaxel concentration in the tumor cells to improve the antitumor activity of this combination.
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spelling pubmed-51042132016-11-28 Evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells Liu, Zeng Zhu, Yuan-Yuan Li, Zhao-Yuan Ning, Si-Qing Oncol Lett Articles The aim of the present study was to evaluate the efficacy of paclitaxel combined with curcumin (CUR) against drug resistance in ovarian cancer cells. PLGA-phospholipid-PEG nanoparticles were prepared using the nano precipitation method. The size and morphology of the nanoparticles were determined using a transmission electron microscope and particle size analyzer. The encapsulation efficiency of nanoparticles was determined using the ultrafiltration centrifugation method. The dialysis method was used to study the release of PLGA-phospholipid-PEG nanoparticles. ADM was used to induce the A2780 cell line (human ovarian cancer cell line) to establish the model of the multidrug-resistant (MDR) cell line, and the protein activity of P-glycoprotein (P-gp) in the A2780 cell line and A2780/ADM resistant cell line was determined using western blot analysis. The results showed that, the prepared nanoparticles were uniform in size, with a size of approximately 100 nm, and round in shape. Additionally, the nanoparticles had a more gentle and slow release than the free drug release. The results of the protein trace printing experiment showed that the P-gp content of the drug-resistant cell line was significantly reduced by the CUR nanoparticles. In conclusion, PLGA-phospholipid nanoparticles containing taxol and CUR have improved solubility and stability together with a slow release effect. In addition, CUR was able to overcome the MDR of tumor cells by elevating the paclitaxel concentration in the tumor cells to improve the antitumor activity of this combination. D.A. Spandidos 2016-11 2016-09-26 /pmc/articles/PMC5104213/ /pubmed/27895754 http://dx.doi.org/10.3892/ol.2016.5192 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Zeng
Zhu, Yuan-Yuan
Li, Zhao-Yuan
Ning, Si-Qing
Evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells
title Evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells
title_full Evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells
title_fullStr Evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells
title_full_unstemmed Evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells
title_short Evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells
title_sort evaluation of the efficacy of paclitaxel with curcumin combination in ovarian cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104213/
https://www.ncbi.nlm.nih.gov/pubmed/27895754
http://dx.doi.org/10.3892/ol.2016.5192
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