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A Nested PCR Assay to Avoid False Positive Detection of the Microsporidian Enterocytozoon hepatopenaei (EHP) in Environmental Samples in Shrimp Farms

Hepatopancreatic microsporidiosis (HPM) caused by Enterocytozoon hepatopenaei (EHP) is an important disease of cultivated shrimp. Heavy infections may lead to retarded growth and unprofitable harvests. Existing PCR detection methods target the EHP small subunit ribosomal RNA (SSU rRNA) gene (SSU-PCR...

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Detalles Bibliográficos
Autores principales: Jaroenlak, Pattana, Sanguanrut, Piyachat, Williams, Bryony A. P., Stentiford, Grant D., Flegel, Timothy W., Sritunyalucksana, Kallaya, Itsathitphaisarn, Ornchuma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104377/
https://www.ncbi.nlm.nih.gov/pubmed/27832178
http://dx.doi.org/10.1371/journal.pone.0166320