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Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses

Although innate color preference of motile organisms may provide clues to behavioral biases, it has remained a longstanding question. In this study, we investigated innate color preference of zebrafish larvae. A cross maze with different color sleeves around each arm was used for the color preferenc...

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Autores principales: Park, Jong-Su, Ryu, Jae-Ho, Choi, Tae-Ik, Bae, Young-Ki, Lee, Suman, Kang, Hae Jin, Kim, Cheol-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104883/
https://www.ncbi.nlm.nih.gov/pubmed/27802373
http://dx.doi.org/10.14348/molcells.2016.0173
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author Park, Jong-Su
Ryu, Jae-Ho
Choi, Tae-Ik
Bae, Young-Ki
Lee, Suman
Kang, Hae Jin
Kim, Cheol-Hee
author_facet Park, Jong-Su
Ryu, Jae-Ho
Choi, Tae-Ik
Bae, Young-Ki
Lee, Suman
Kang, Hae Jin
Kim, Cheol-Hee
author_sort Park, Jong-Su
collection PubMed
description Although innate color preference of motile organisms may provide clues to behavioral biases, it has remained a longstanding question. In this study, we investigated innate color preference of zebrafish larvae. A cross maze with different color sleeves around each arm was used for the color preference test (R; red, G; green, B; blue, Y; yellow). The findings showed that 5 dpf zebrafish larvae preferred blue over other colors (B > R > G > Y). To study innate color recognition further, tyrosinase mutants were generated using CRISPR/Cas9 system. As a model for oculocutaneous albinism (OCA) and color vision impairment, tyrosinase mutants demonstrated diminished color sensation, indicated mainly by hypopigmentation of the retinal pigment epithelium (RPE). Due to its relative simplicity and ease, color preference screening using zebrafish larvae is suitable for high-throughput screening applications. This system may potentially be applied to the analysis of drug effects on larval behavior or the detection of sensory deficits in neurological disorder models, such as autism-related disorders, using mutant larvae generated by the CRISPR/Cas9 technique.
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spelling pubmed-51048832016-12-01 Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses Park, Jong-Su Ryu, Jae-Ho Choi, Tae-Ik Bae, Young-Ki Lee, Suman Kang, Hae Jin Kim, Cheol-Hee Mol Cells Article Although innate color preference of motile organisms may provide clues to behavioral biases, it has remained a longstanding question. In this study, we investigated innate color preference of zebrafish larvae. A cross maze with different color sleeves around each arm was used for the color preference test (R; red, G; green, B; blue, Y; yellow). The findings showed that 5 dpf zebrafish larvae preferred blue over other colors (B > R > G > Y). To study innate color recognition further, tyrosinase mutants were generated using CRISPR/Cas9 system. As a model for oculocutaneous albinism (OCA) and color vision impairment, tyrosinase mutants demonstrated diminished color sensation, indicated mainly by hypopigmentation of the retinal pigment epithelium (RPE). Due to its relative simplicity and ease, color preference screening using zebrafish larvae is suitable for high-throughput screening applications. This system may potentially be applied to the analysis of drug effects on larval behavior or the detection of sensory deficits in neurological disorder models, such as autism-related disorders, using mutant larvae generated by the CRISPR/Cas9 technique. Korean Society for Molecular and Cellular Biology 2016-10-31 2016-10-31 /pmc/articles/PMC5104883/ /pubmed/27802373 http://dx.doi.org/10.14348/molcells.2016.0173 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
spellingShingle Article
Park, Jong-Su
Ryu, Jae-Ho
Choi, Tae-Ik
Bae, Young-Ki
Lee, Suman
Kang, Hae Jin
Kim, Cheol-Hee
Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses
title Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses
title_full Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses
title_fullStr Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses
title_full_unstemmed Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses
title_short Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses
title_sort innate color preference of zebrafish and its use in behavioral analyses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5104883/
https://www.ncbi.nlm.nih.gov/pubmed/27802373
http://dx.doi.org/10.14348/molcells.2016.0173
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