Cargando…

Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals

The ocular surface is strongly affected by oxidative stress, which causes many ocular diseases including dry eye. Previously, we showed that selenium compounds, e.g., selenoprotein P and Se-lactoferrin, were candidates for treatment of dry eye. This paper shows the efficacy of Se-lactoferrin for the...

Descripción completa

Detalles Bibliográficos
Autores principales: Higuchi, Akihiro, Inoue, Hiroyoshi, Kaneko, Yoshio, Oonishi, Erina, Tsubota, Kazuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5105079/
https://www.ncbi.nlm.nih.gov/pubmed/27833152
http://dx.doi.org/10.1038/srep36903
_version_ 1782466831263465472
author Higuchi, Akihiro
Inoue, Hiroyoshi
Kaneko, Yoshio
Oonishi, Erina
Tsubota, Kazuo
author_facet Higuchi, Akihiro
Inoue, Hiroyoshi
Kaneko, Yoshio
Oonishi, Erina
Tsubota, Kazuo
author_sort Higuchi, Akihiro
collection PubMed
description The ocular surface is strongly affected by oxidative stress, which causes many ocular diseases including dry eye. Previously, we showed that selenium compounds, e.g., selenoprotein P and Se-lactoferrin, were candidates for treatment of dry eye. This paper shows the efficacy of Se-lactoferrin for the treatment of dry eye compared with Diquas as a control drug using two dry eye models and incorporation of lactoferrin into corneal epithelial cells via lactoferrin receptors. We show the efficacy of Se-lactoferrin eye drops in the tobacco smoke exposure rat dry eye model and short-term rabbit dry eye model, although Diquas eye drops were only effective in the short-term rabbit dry eye model. These results indicate that Se-lactoferrin was useful in the oxidative stress-causing dry eye model. Se-lactoferrin was taken into corneal epithelium cells via lactoferrin receptors. We identified LRP1 as the lactoferrin receptor in the corneal epithelium involved in lactoferrin uptake. Se-lactoferrin eye drops did not irritate the ocular surface of rabbits. Se-lactoferrin was an excellent candidate for treatment of dry eye, reducing oxidative stress by a novel mechanism.
format Online
Article
Text
id pubmed-5105079
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-51050792016-11-17 Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals Higuchi, Akihiro Inoue, Hiroyoshi Kaneko, Yoshio Oonishi, Erina Tsubota, Kazuo Sci Rep Article The ocular surface is strongly affected by oxidative stress, which causes many ocular diseases including dry eye. Previously, we showed that selenium compounds, e.g., selenoprotein P and Se-lactoferrin, were candidates for treatment of dry eye. This paper shows the efficacy of Se-lactoferrin for the treatment of dry eye compared with Diquas as a control drug using two dry eye models and incorporation of lactoferrin into corneal epithelial cells via lactoferrin receptors. We show the efficacy of Se-lactoferrin eye drops in the tobacco smoke exposure rat dry eye model and short-term rabbit dry eye model, although Diquas eye drops were only effective in the short-term rabbit dry eye model. These results indicate that Se-lactoferrin was useful in the oxidative stress-causing dry eye model. Se-lactoferrin was taken into corneal epithelium cells via lactoferrin receptors. We identified LRP1 as the lactoferrin receptor in the corneal epithelium involved in lactoferrin uptake. Se-lactoferrin eye drops did not irritate the ocular surface of rabbits. Se-lactoferrin was an excellent candidate for treatment of dry eye, reducing oxidative stress by a novel mechanism. Nature Publishing Group 2016-11-11 /pmc/articles/PMC5105079/ /pubmed/27833152 http://dx.doi.org/10.1038/srep36903 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Higuchi, Akihiro
Inoue, Hiroyoshi
Kaneko, Yoshio
Oonishi, Erina
Tsubota, Kazuo
Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals
title Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals
title_full Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals
title_fullStr Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals
title_full_unstemmed Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals
title_short Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals
title_sort selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5105079/
https://www.ncbi.nlm.nih.gov/pubmed/27833152
http://dx.doi.org/10.1038/srep36903
work_keys_str_mv AT higuchiakihiro seleniumbindinglactoferrinistakenintocornealepithelialcellsbyareceptorandpreventscornealdamageindryeyemodelanimals
AT inouehiroyoshi seleniumbindinglactoferrinistakenintocornealepithelialcellsbyareceptorandpreventscornealdamageindryeyemodelanimals
AT kanekoyoshio seleniumbindinglactoferrinistakenintocornealepithelialcellsbyareceptorandpreventscornealdamageindryeyemodelanimals
AT oonishierina seleniumbindinglactoferrinistakenintocornealepithelialcellsbyareceptorandpreventscornealdamageindryeyemodelanimals
AT tsubotakazuo seleniumbindinglactoferrinistakenintocornealepithelialcellsbyareceptorandpreventscornealdamageindryeyemodelanimals