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12-months metabolic changes among gender dysphoric individuals under cross-sex hormone treatment: a targeted metabolomics study
Metabolomic analyses in epidemiological studies have demonstrated a strong sexual dimorphism for most metabolites. Cross-sex hormone treatment (CSH) in transgender individuals enables the study of metabolites in a cross-gender setting. Targeted metabolomic profiling of serum of fasting transmen and...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5105120/ https://www.ncbi.nlm.nih.gov/pubmed/27833161 http://dx.doi.org/10.1038/srep37005 |
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author | Auer, Matthias K. Cecil, Alexander Roepke, Yasmin Bultynck, Charlotte Pas, Charlotte Fuss, Johannes Prehn, Cornelia Wang-Sattler, Rui Adamski, Jerzy Stalla, Günter K. T’Sjoen, Guy |
author_facet | Auer, Matthias K. Cecil, Alexander Roepke, Yasmin Bultynck, Charlotte Pas, Charlotte Fuss, Johannes Prehn, Cornelia Wang-Sattler, Rui Adamski, Jerzy Stalla, Günter K. T’Sjoen, Guy |
author_sort | Auer, Matthias K. |
collection | PubMed |
description | Metabolomic analyses in epidemiological studies have demonstrated a strong sexual dimorphism for most metabolites. Cross-sex hormone treatment (CSH) in transgender individuals enables the study of metabolites in a cross-gender setting. Targeted metabolomic profiling of serum of fasting transmen and transwomen at baseline and following 12 months of CSH (N = 20/group) was performed. Changes in 186 serum metabolites and metabolite ratios were determined by targeted metabolomics analysis based on ESI-LC-MS/MS. RandomForest (RF) analysis was applied to detect metabolites of highest interest for grouping of transwomen and transmen before and after initiation of CSH. Principal component analysis (PCA) was performed to check whether group differentiation was achievable according to these variables and to see if changes in metabolite levels could be explained by a priori gender differences. PCA predicted grouping of individuals-determined by the citrulline/arginine-ratio and the amino acids lysine, alanine and asymmetric dimethylarginine - in addition to the expected grouping due to changes in sex steroids and body composition. The fact that most of the investigated metabolites did, however, not change, indicates that the majority of sex dependent differences in metabolites reported in the literature before may primarily not be attributable to sex hormones but to other gender-differences. |
format | Online Article Text |
id | pubmed-5105120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51051202016-11-17 12-months metabolic changes among gender dysphoric individuals under cross-sex hormone treatment: a targeted metabolomics study Auer, Matthias K. Cecil, Alexander Roepke, Yasmin Bultynck, Charlotte Pas, Charlotte Fuss, Johannes Prehn, Cornelia Wang-Sattler, Rui Adamski, Jerzy Stalla, Günter K. T’Sjoen, Guy Sci Rep Article Metabolomic analyses in epidemiological studies have demonstrated a strong sexual dimorphism for most metabolites. Cross-sex hormone treatment (CSH) in transgender individuals enables the study of metabolites in a cross-gender setting. Targeted metabolomic profiling of serum of fasting transmen and transwomen at baseline and following 12 months of CSH (N = 20/group) was performed. Changes in 186 serum metabolites and metabolite ratios were determined by targeted metabolomics analysis based on ESI-LC-MS/MS. RandomForest (RF) analysis was applied to detect metabolites of highest interest for grouping of transwomen and transmen before and after initiation of CSH. Principal component analysis (PCA) was performed to check whether group differentiation was achievable according to these variables and to see if changes in metabolite levels could be explained by a priori gender differences. PCA predicted grouping of individuals-determined by the citrulline/arginine-ratio and the amino acids lysine, alanine and asymmetric dimethylarginine - in addition to the expected grouping due to changes in sex steroids and body composition. The fact that most of the investigated metabolites did, however, not change, indicates that the majority of sex dependent differences in metabolites reported in the literature before may primarily not be attributable to sex hormones but to other gender-differences. Nature Publishing Group 2016-11-11 /pmc/articles/PMC5105120/ /pubmed/27833161 http://dx.doi.org/10.1038/srep37005 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Auer, Matthias K. Cecil, Alexander Roepke, Yasmin Bultynck, Charlotte Pas, Charlotte Fuss, Johannes Prehn, Cornelia Wang-Sattler, Rui Adamski, Jerzy Stalla, Günter K. T’Sjoen, Guy 12-months metabolic changes among gender dysphoric individuals under cross-sex hormone treatment: a targeted metabolomics study |
title | 12-months metabolic changes among gender dysphoric individuals under cross-sex hormone treatment: a targeted metabolomics study |
title_full | 12-months metabolic changes among gender dysphoric individuals under cross-sex hormone treatment: a targeted metabolomics study |
title_fullStr | 12-months metabolic changes among gender dysphoric individuals under cross-sex hormone treatment: a targeted metabolomics study |
title_full_unstemmed | 12-months metabolic changes among gender dysphoric individuals under cross-sex hormone treatment: a targeted metabolomics study |
title_short | 12-months metabolic changes among gender dysphoric individuals under cross-sex hormone treatment: a targeted metabolomics study |
title_sort | 12-months metabolic changes among gender dysphoric individuals under cross-sex hormone treatment: a targeted metabolomics study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5105120/ https://www.ncbi.nlm.nih.gov/pubmed/27833161 http://dx.doi.org/10.1038/srep37005 |
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