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Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation

BACKGROUND: Porcine circovirus 2 causes different clinical syndromes resulting in a significant economic loss in the pork industry. Three pigs with unexplained cardiac and multi-organ inflammation that tested negative for PCV2 and other known porcine pathogens were further analyzed. METHODS: Histolo...

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Autores principales: Phan, Tung Gia, Giannitti, Federico, Rossow, Stephanie, Marthaler, Douglas, Knutson, Todd, Li, Linlin, Deng, Xutao, Resende, Talita, Vannucci, Fabio, Delwart, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5105309/
https://www.ncbi.nlm.nih.gov/pubmed/27835942
http://dx.doi.org/10.1186/s12985-016-0642-z
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author Phan, Tung Gia
Giannitti, Federico
Rossow, Stephanie
Marthaler, Douglas
Knutson, Todd
Li, Linlin
Deng, Xutao
Resende, Talita
Vannucci, Fabio
Delwart, Eric
author_facet Phan, Tung Gia
Giannitti, Federico
Rossow, Stephanie
Marthaler, Douglas
Knutson, Todd
Li, Linlin
Deng, Xutao
Resende, Talita
Vannucci, Fabio
Delwart, Eric
author_sort Phan, Tung Gia
collection PubMed
description BACKGROUND: Porcine circovirus 2 causes different clinical syndromes resulting in a significant economic loss in the pork industry. Three pigs with unexplained cardiac and multi-organ inflammation that tested negative for PCV2 and other known porcine pathogens were further analyzed. METHODS: Histology was used to identify microscopic lesions in multiple tissues. Metagenomics was used to detect viral sequences in tissue homogenates. In situ hybridization was used to detect viral RNA expression in cardiac tissue. RESULTS: In all three cases we characterized the genome of a new circovirus we called PCV3 with a replicase and capsid proteins showing 55 and 35 % identities to the genetically-closest proteins from a bat-feces associated circovirus and were even more distant to those of porcine circovirus 1 and 2. Common microscopic lesions included non-suppurative myocarditis and/or cardiac arteriolitis. Viral mRNA was detected intralesionally in cardiac cells. Deep sequencing in tissues also revealed the presence of porcine astrovirus 4 in all three animals as well as rotavirus A, porcine cytomegalovirus and porcine hemagglutinating encephalomyelitis virus in individual cases. CONCLUSION: The pathogenicity and molecular epidemiology of this new circovirus, alone or in the context of co-infections, warrants further investigations.
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spelling pubmed-51053092016-11-14 Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation Phan, Tung Gia Giannitti, Federico Rossow, Stephanie Marthaler, Douglas Knutson, Todd Li, Linlin Deng, Xutao Resende, Talita Vannucci, Fabio Delwart, Eric Virol J Short Report BACKGROUND: Porcine circovirus 2 causes different clinical syndromes resulting in a significant economic loss in the pork industry. Three pigs with unexplained cardiac and multi-organ inflammation that tested negative for PCV2 and other known porcine pathogens were further analyzed. METHODS: Histology was used to identify microscopic lesions in multiple tissues. Metagenomics was used to detect viral sequences in tissue homogenates. In situ hybridization was used to detect viral RNA expression in cardiac tissue. RESULTS: In all three cases we characterized the genome of a new circovirus we called PCV3 with a replicase and capsid proteins showing 55 and 35 % identities to the genetically-closest proteins from a bat-feces associated circovirus and were even more distant to those of porcine circovirus 1 and 2. Common microscopic lesions included non-suppurative myocarditis and/or cardiac arteriolitis. Viral mRNA was detected intralesionally in cardiac cells. Deep sequencing in tissues also revealed the presence of porcine astrovirus 4 in all three animals as well as rotavirus A, porcine cytomegalovirus and porcine hemagglutinating encephalomyelitis virus in individual cases. CONCLUSION: The pathogenicity and molecular epidemiology of this new circovirus, alone or in the context of co-infections, warrants further investigations. BioMed Central 2016-11-11 /pmc/articles/PMC5105309/ /pubmed/27835942 http://dx.doi.org/10.1186/s12985-016-0642-z Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Phan, Tung Gia
Giannitti, Federico
Rossow, Stephanie
Marthaler, Douglas
Knutson, Todd
Li, Linlin
Deng, Xutao
Resende, Talita
Vannucci, Fabio
Delwart, Eric
Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation
title Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation
title_full Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation
title_fullStr Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation
title_full_unstemmed Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation
title_short Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation
title_sort detection of a novel circovirus pcv3 in pigs with cardiac and multi-systemic inflammation
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5105309/
https://www.ncbi.nlm.nih.gov/pubmed/27835942
http://dx.doi.org/10.1186/s12985-016-0642-z
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