High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform

A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC(50) values between undifferentiated and dif...

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Detalles Bibliográficos
Autores principales: Nierode, Gregory J., Perea, Brian C., McFarland, Sean K., Pascoal, Jorge F., Clark, Douglas S., Schaffer, David V., Dordick, Jonathan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5106528/
https://www.ncbi.nlm.nih.gov/pubmed/28157485
http://dx.doi.org/10.1016/j.stemcr.2016.10.001
Descripción
Sumario:A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC(50) values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.

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