High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform

A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC(50) values between undifferentiated and dif...

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Autores principales: Nierode, Gregory J., Perea, Brian C., McFarland, Sean K., Pascoal, Jorge F., Clark, Douglas S., Schaffer, David V., Dordick, Jonathan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5106528/
https://www.ncbi.nlm.nih.gov/pubmed/28157485
http://dx.doi.org/10.1016/j.stemcr.2016.10.001
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author Nierode, Gregory J.
Perea, Brian C.
McFarland, Sean K.
Pascoal, Jorge F.
Clark, Douglas S.
Schaffer, David V.
Dordick, Jonathan S.
author_facet Nierode, Gregory J.
Perea, Brian C.
McFarland, Sean K.
Pascoal, Jorge F.
Clark, Douglas S.
Schaffer, David V.
Dordick, Jonathan S.
author_sort Nierode, Gregory J.
collection PubMed
description A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC(50) values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.
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spelling pubmed-51065282016-11-17 High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform Nierode, Gregory J. Perea, Brian C. McFarland, Sean K. Pascoal, Jorge F. Clark, Douglas S. Schaffer, David V. Dordick, Jonathan S. Stem Cell Reports Article A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC(50) values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells. Elsevier 2016-10-27 /pmc/articles/PMC5106528/ /pubmed/28157485 http://dx.doi.org/10.1016/j.stemcr.2016.10.001 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nierode, Gregory J.
Perea, Brian C.
McFarland, Sean K.
Pascoal, Jorge F.
Clark, Douglas S.
Schaffer, David V.
Dordick, Jonathan S.
High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform
title High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform
title_full High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform
title_fullStr High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform
title_full_unstemmed High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform
title_short High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform
title_sort high-throughput toxicity and phenotypic screening of 3d human neural progenitor cell cultures on a microarray chip platform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5106528/
https://www.ncbi.nlm.nih.gov/pubmed/28157485
http://dx.doi.org/10.1016/j.stemcr.2016.10.001
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