Cargando…

Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

BACKGROUND: The Luminex bead-based multiplex assay is useful for quantifying immune mediators such as cytokines and chemokines. Cross-comparisons of reagents for this technique from different suppliers have already been performed using serum or plasma but rarely with supernatants collected from anti...

Descripción completa

Detalles Bibliográficos
Autores principales:	Surenaud, Mathieu, Manier, Céline, Richert, Laura, Thiébaut, Rodolphe, Levy, Yves, Hue, Sophie, Lacabaratz, Christine
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5106791/ https://www.ncbi.nlm.nih.gov/pubmed/27835944 http://dx.doi.org/10.1186/s12865-016-0182-8

Ejemplares similares

Decreased HIV-Specific T-Regulatory Responses Are Associated with Effective DC-Vaccine Induced Immunity
por: Brezar, Vedran, et al.
Publicado: (2015)

Accelerating clinical development of HIV vaccine strategies: methodological challenges and considerations in constructing an optimised multi-arm phase I/II trial design
por: Richert, Laura, et al.
Publicado: (2014)

Evaluation of cytokines in peripheral blood mononuclear cell supernatants for the diagnosis of tuberculosis
por: Sariko, Margaretha, et al.
Publicado: (2018)

Optimization of a magnetic bead-based assay (MAGPIX(®)-Luminex) for immune surveillance of exposure to malaria using multiple Plasmodium antigens and sera from different endemic settings
por: Varela, Marie Louise, et al.
Publicado: (2018)

Rapid detection of three rabbit pathogens by use of the Luminex x-TAG assay
por: Wu, Miaoli, et al.
Publicado: (2018)

Development of a sensitive Luminex xMAP-based microsphere immunoassay for specific detection of Iris yellow spot virus
por: Yu, Cui, et al.
Publicado: (2018)

Immune Alterations in a Patient with SARS-CoV-2-Related Acute Respiratory Distress Syndrome
por: Bouadma, Lila, et al.
Publicado: (2020)

Gene Expression Signatures Associated With Immune and Virological Responses to Therapeutic Vaccination With Dendritic Cells in HIV-Infected Individuals
por: Thiébaut, Rodolphe, et al.
Publicado: (2019)

Negative modulation of suppressive HIV-specific regulatory T cells by IL-2 adjuvanted therapeutic vaccine
por: Brezar, Vedran, et al.
Publicado: (2017)

Detection of tumor-associated cells in cryopreserved peripheral blood mononuclear cell samples for retrospective analysis
por: Zhu, Peixuan, et al.
Publicado: (2016)

IL-7 abrogates memory T regulatory cell functions by modulation of CD39/ATP axis in vitro and in vivo in HIV infected and non-infected patients
por: Younas, M, et al.
Publicado: (2012)

Purification of viable peripheral blood mononuclear cells for biobanking using a robotized liquid handling workstation
por: Coppola, Luigi, et al.
Publicado: (2019)

ALG: Automated Genotype Calling of Luminex Assays
por: Bourgey, Mathieu, et al.
Publicado: (2011)

Supernatant of Bone Marrow Mesenchymal Stromal Cells Induces Peripheral Blood Mononuclear Cells Possessing Mesenchymal Features
por: Hu, Gang, et al.
Publicado: (2011)

Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells
por: Monleau, Marjorie, et al.
Publicado: (2014)

Direct analysis of mAb aggregates in mammalian cell culture supernatant
por: Paul, Albert J, et al.
Publicado: (2014)

Anti-HIV potency of T-cell responses elicited by dendritic cell therapeutic vaccination
por: Surenaud, Mathieu, et al.
Publicado: (2019)

Methodological guidelines to estimate population-based health indicators using linked data and/or machine learning techniques
por: Haneef, Romana, et al.
Publicado: (2022)

Durable natural killer cell responses after heterologous two-dose Ebola vaccination
por: Wagstaffe, Helen R., et al.
Publicado: (2021)

NK Cell Subset Redistribution and Antibody Dependent Activation after Ebola Vaccination in Africans
por: Wagstaffe, Helen R., et al.
Publicado: (2022)

Transcriptional analysis of human peripheral blood mononuclear cells stimulated by Mycobacterium tuberculosis antigen
por: Wei, Jing, et al.
Publicado: (2023)

Impact of Immunoglobulin M-Type Donor-Specific Human Leukocyte Antigen–Antibody Levels in Supernatants from Cultured Peripheral Blood Mononuclear Cells as Predictors of Antibody-Mediated Rejection
por: Imamura, Ryoichi, et al.
Publicado: (2020)

Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate
por: Singh, Balwan, et al.
Publicado: (2021)

A novel approach for biomarker selection and the integration of repeated measures experiments from two assays
por: Liquet, Benoit, et al.
Publicado: (2012)

Design, immunogenicity, and efficacy of a pan-sarbecovirus dendritic-cell targeting vaccine
por: Coléon, Séverin, et al.
Publicado: (2022)

NOD-scid IL2R γ(null) mice engrafted with human peripheral blood mononuclear cells as a model to test therapeutics targeting human signaling pathways
por: Zadeh-Khorasani, Maryam, et al.
Publicado: (2013)

HIV-1 T cell epitopes targeted to Rhesus macaque CD40 and DCIR: A comparative study of prototype dendritic cell targeting therapeutic vaccine candidates
por: Flamar, Anne-Laure, et al.
Publicado: (2018)

Mathematical modelling of the automated FADU assay for the quantification of DNA strand breaks and their repair in human peripheral mononuclear blood cells
por: Junk, Michael, et al.
Publicado: (2014)

Single cell transcriptome sequencing of stimulated and frozen human peripheral blood mononuclear cells
por: Derbois, Céline, et al.
Publicado: (2023)

Supernatants from lymphocytes stimulated with Bacillus Calmette-Guerin can modify the antigenicity of tumours and stimulate allogeneic T-cell responses
por: Liu, W M, et al.
Publicado: (2011)

CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death
por: Lévy, Yves, et al.
Publicado: (2021)

Modelling the response to vaccine in non-human primates to define SARS-CoV-2 mechanistic correlates of protection
por: Alexandre, Marie, et al.
Publicado: (2022)

CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death
por: Lévy, Yves, et al.
Publicado: (2022)

Subset- and Antigen-Specific Effects of Treg on CD8+ T Cell Responses in Chronic HIV Infection
por: Nikolova, Maria, et al.
Publicado: (2016)

Quantifying and Predicting the Effect of Exogenous Interleukin-7 on CD4(+)T Cells in HIV-1 Infection
por: Thiébaut, Rodolphe, et al.
Publicado: (2014)

Psp140: an immunodominant antigen in the supernatant of Streptococcus pneumoniae culture
por: Afshar, Davoud, et al.
Publicado: (2020)

Tear Luminex Analysis in Dry Eye Patients
por: Zhao, Hailan, et al.
Publicado: (2018)

Impact of sensitivity of human leucocyte antigen antibody detection by Luminex technology on graft loss at 1 year
por: Szatmary, Peter, et al.
Publicado: (2013)

A Randomized Placebo-Controlled Efficacy Study of a Prime Boost Therapeutic Vaccination Strategy in HIV-1-Infected Individuals: VRI02 ANRS 149 LIGHT Phase II Trial
por: Lévy, Y., et al.
Publicado: (2021)

No Positive Association between Vitamin D Level and Immune Responses to Hepatitis B and Streptococcus pneumoniae Vaccination in HIV-Infected Adults
por: Viard, Jean-Paul, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_hrkn30m9j9hi0fqoougu5rt1cl